An optode was designed for an indirect determination of ascorbic acid by using neocuproine, which has been coated on transparent triacetylcellulose film as a membrane. The proposed method is based on the oxidation of ascorbic acid to dehydroascorbic acid with the Cu(II) and neocuproine reagent. The increase in the absorbance value of the optode at the maximum wavelength of 455 nm was related ...

the migration of aluminum (al) from aseptic laminated paperboard packages was studied by monitoring the al content of orange juice (plain juice, no additives) filled into such packages and stored at ambient temperature (23ºc) for up to 1 year. analytical procedure includes mw-assisted digestion of orange juice with concentrated nitric acid followed by determination of al concentration by double-...

The oranges were purchased from the local market of Bela. The orange peel and pulp were subjected to successive extraction with solvents in increasing order of their polarity viz. Acetone, hexane, methanol and distilled water. Orange peel and pulp powder was extracted separately by aqueous extraction. The phenolic content of these samples were studied according to the method described by Folin ...

Backgrounds and Aims: The purpose of this study was to examine the effects of potassium, sodium and zinc metal ions on ammonium sulfate fraction of acid phosphatase activity of orange fruit. Materials and Methods: Acid phosphatase was extracted and purified from orange fruit and then various samples as well as a control sample were prepared in vitro. In addition to substrates, ions of potass...

Acridine orange is a metachromatic intercalator used extensively in histochemistry to differentiate double- from single-stranded (ds, ss) nucleic acid by the emission of green and red fluorescence, respectively, under ultraviolet light. In the present study we standardised a protocol in order to use acridine orange to detect rotavirus ds RNA in polyacrylamide gels and compared it to silver and ...

introduction: citrus fruits, which belong to the family of rutaceae are one of the main fruit tree crops grown throughout the world. although sweet orange (citrus sinensis) is the major fruit in this group accounting for about 70% of citrus output.citrus fruits are well – appreciated fortheir refreshing juice and health benefits.the health benefits of citrus fruits are linked to the high amount...

  Abstract: Spectrophotometric studies were successfully used in quantitative analysis of Amitriptyline Hydrochloride (ATH) Two new methods using spectrophotometry are described for the determination of (ATH) with potassium bromate as the oxidizing agent and acid dyes, Methyl orange and Indigo carmine. Both spectrophotometric methods are based o...

Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...