نتایج جستجو برای: acid phosphatase
تعداد نتایج: 784468 فیلتر نتایج به سال:
BACKGROUND Bone changes are common in sickle cell disease, but the pathogenesis is not fully understood. Tartrate-resistant acid phosphatase (TRACP) type 5b is produced by bone-resorbing osteoclasts. In other forms of hemolytic anemia, increased iron stores are associated with osteoporosis. We hypothesized that transfusional iron overload would be associated with increased osteoclast activity i...
phage elisa is a common method to confirm binding of obtained phages from phage display technique to related antigens. enzyme-conjugated antibody directed against the major capsid protein (pviii) or enzyme-conjugated secondary antibody against the primary antibody is used as a detection system in phage elisa. we have suggested to express an acid phosphatase (sapm) enzyme on m13 pix minor coat p...
To explain the different secretion kinetics of lysosomal enzymes in Dictyostelium discoideum, previous investigators have hypothesized the existence of a heterogeneous population of lysosomes containing either the enzyme acid phosphatase or other hydrolase enzymes. This proposal predicts that at least two targeting mechanisms exist for lysosomal enzymes in this organism. To begin to investigate...
5. Warren RJ, Moss DW. An automated continuous-monitoring procedure for the determination of acid phosphatase activity in serum. Clin Chim Acta 77, 179-188 (1977). 6. Gundlach G, Muhlhausen B. Untersuchungen zur Kupplung des 1-Naphthols mit Fast-red-TR. J Clin Chem Clin Biochem 18, 603-610 (1980). 7. Babson AL, Read PA. A new assay for prostatic acid phosphatase in serum. Am J Clin Pathol 32, 8...
One of the cyr 1 mutants (cyr 1-2) in yeast produced low levels of adenylate cyclase and cyclic AMP at 25 degrees and was unable to derepress acid phosphatase. Addition of cyclic AMP to the cyr1-2 cultures elevated the level of repressible acid phosphatase activity. The bcy1 mutation, which suppresses the cyr1-2 mutation by allowing activity of a cyclic AMP-independent protein kinase, also allo...
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