Adenine transport in the L1210 leukemia cell is mediated by a rapid facilitated process. Evidence supporting a carrier transport mechanism includes the observations that 1) extracellular hypoxanthine or xanthine inhibits net adenine uptake and 2) the presence of these bases within the intracellular compartment produces an uphill flow of adenine into the cell (countertransport). On the other han...

It is well-established that there is a crosstalk between the lung and the kidney, and several studies have reported association between chronic kidney disease (CKD) and pulmonary pathophysiological changes. Experimentally, CKD can be caused in mice by dietary intake of adenine. Nevertheless, the consequence of such intervention on the lung received only scant attention. Here, we assessed the pu...

The AMP-activated protein kinase (AMPK) signaling system plays a key role in cellular stress by repressing the inflammatory responses induced by the nuclear factor-kappa B (NF-κB) system. Previous studies suggest that the anti-inflammatory role of AMPK involves activation by adenine, but the mechanism that allows adenine to produce these effects has not yet been elucidated. In human umbilical v...

The mechanism of adenine toxicity in an hpt gpt strain of Escherichia coli that is extremely sensitive to adenine inhibition was investigated. Adenine-resistant derivatives had secondary mutations in adeninephosphoribosyltransferase or the purR repressor. Growth studies with various purine salvage pathway mutants and the ability of guanosine to prevent adenine toxicity indicated that adenine ex...

Smith, Robert C. (Auburn University, Auburn. Ala.), and W. D. Salmon. Enhancement by adenine of the inhibition of Salmonella typhimurium by ethionine. J. Bacteriol. 89:1494-1498. 1965.-The inhibition by ethionine of the growth of Salmonella typhimurium was increased by adenine and several other purines. This effect was counteracted by the addition of methionine to the cultures. There was no pre...

We have developed a novel chemistry-based method to detect adenine methylation. A DNA probe functionalized with a formyl group discriminates between adenine and N6-methyl adenine by the formation of a selective interstrand cross-link.

The metabolism and intracellular compartmentalization of adenine nucleotides in a preparation of adult rat heart myocytes showing good morphology, viability, and tolerance to calcium ion has been examined by high performance liquid chromatography. These myocytes contain an average of 23 nmol adenine nucleotide per milligram protein which is about 60% of the adenine nucleotide content of intact ...