In order to characterize a step in the reaction sequence of sodium and potassium ion-dependent adenosine triphosphatase (EC 3.6.1.3), the binding of radioactive adenosine triphosphate to kidney cell membranes was estimated by flow dialysis in the presence of a chelator of divalent cations, which prevented hydrolysis of the adenosine triphosphate. Adenosine triphosphate was bound to a single sit...

Nicotinamide adenine dinucleotide (NAD) oscillation was observed when the isolated mitochondria were immersed in a pyruvate solution. In addition, an adenosine diphosphate (ADP) added to mitochondrial suspension containing pyruvate, triphosphate (ATP) as well NADH oscillation. At this time, pH within also oscillated. It found that oscillatory reaction of caused by membrane permeation continues,...

In this study, the conformational structure of tetra-protonated Adenosine 5’triphosphate molecule has been investigated using Spartan 08 package program with molecular dynamics method. Following analysis; geometry optimization, excited states, absorption and fluorescence (emission) spectra, orbitals, chemical hardness, electronic potential, electronegativity electrophilicity indexes were calcul...

Heat shock protein 90 (Hsp90) is a molecular chaperone that assists folding in an Adenosine triphosphate (ATP)-dependent way. Hsp90 has been reported to interact with Alzheimeŕs disease associated amyloid-β (Aβ) peptides and suppress toxic oligomer- fibril formation. However, the mechanism remains largely unclear. Here we use combination of atomic force microscopy (AFM) imaging, circular dichro...

Background: Human epidermal growth factor receptor 2 (Her2) gene located in human chromosome17, encodes Her2 tyrosine kinase protein, and is overexpressed breast cancer cells. activated on phosphorylation of by adenosine triphosphate (ATP). Nonetheless, excessively partakes the development prognosis specific types aggressive cancers. Therefore, inhibition therapy primary target for treatment ca...

Abstract Background Ex vivo and in detection imaging of adenosine triphosphate (ATP) is critically important for the diagnosis treatment diseases, which still remains challenges up to present. Results We herein demonstrate that ATP could be fluorescently detected imaged ex vivo. In particular, we fabricate a kind fluorescent probes, are made titanium carbide (TC) nanosheets modified with ROX-ta...

The addition of Ca2+, adenosine 5'-[gamma-thio]triphosphate (ATP[S]) or guanosine 5'-[gamma-thio]triphosphate (GTP[S]) to digitonin-permeabilized PC12 cells stimulates noradrenaline secretion. Both ATP[S] and GTP[S] stimulate release in the absence of Ca2+. Whereas ADP and adenosine 5'-[beta gamma-imido]triphosphate inhibited ATP[S]-stimulated release, they did not inhibit Ca(2+)-stimulated rel...

Purinergic signalling regulates airway defence mechanisms, suggesting that extracellular purines could serve as airway inflammation biomarkers in cystic fibrosis (CF). The purines adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine were measured in sputum from 21 adults (spontaneously expectorated from seven CF patients, induced from 14 healthy...

Two types of turbidimetric detection of adenosine 5'-triphosphate (ATP) by the naked eye were achieved through a combination of non-cross-linking aggregation of DNA-linked polymeric micelles and molecular recognition of ATP by a DNA aptamer.

A highly purified isoenzyme of apyrase obtained from potatoes (Solanum tuberosum var. Pimpernel) exhibits a low specificity for the organic moiety of synthetic pyro- and triphosphates. Methyl di- and tri-phosphates were hydrolysed at higher rates than ADP and ATP, but their Km values were also higher. Steric hindrance at the carbon atom linked to the pyrophosphate chain decreases both binding a...