Molecular cloning of the alpha 2A-adrenergic receptor has shown that this receptor is a member of the gene superfamily of guanine nucleotide-binding protein (G protein)-coupled receptors. The alpha 2A-adrenergic receptor expressed in Chinese hamster ovary (CHO) cells attenuates and potentiates forskolin-stimulated cAMP production through independent signaling pathways. To examine the role of th...

Adrenergic receptor β3 (ADRβ3) is a member of the rhodopsin-like G protein-coupled family. The binding ligand to ADRβ3 activates adenylate cyclase and increases cAMP in cells. highly expressed white brown adipocytes controls key regulatory pathways lipid metabolism. Trp64Arg (W64R) polymorphism associated with early development type 2 diabetes mellitus, lower resting metabolic rate, abdominal o...

A 5-trial inhibitory avoidance test using spontaneously hypertensive rat (SHR) pups has been used as an animal model of attention deficit hyperactivity disorder (ADHD). However, the roles of noradrenergic systems, which are involved in the pathophysiology of ADHD, have not been investigated in this model. In the present study, the effects of adrenergic α2 receptor stimulation, which has been an...

Characterization of adrenergic receptors in membranes from the rat seminal vesicle was studied by radioligand binding assay. Seminal vesicle membranes contained saturable and high affinity binding sites for the beta-adrenergic receptor antagonist 3H-dihydroalprenolol (3H-DHA) and for the alpha-adrenergic antagonist 3H-prazosin. The observed order of potency for adrenergic agonists in competing ...

The adenylate cyclase-coupled &-adrenergic receptor of the frog erythrocyte has served as a useful model system for elucidating the mechanisms of catecholamine-induced desensitization. In this system, it has been previously demonstrated that agonist-induced refractoriness is associated with sequestration of the @adrenergic receptors in vesicles away from the cell surface and from their effector...

The potential role of adrenergic systems in regulating Na(+) uptake in zebrafish (Danio rerio) larvae was investigated. Treatment with isoproterenol (a generic β-adrenergic receptor agonist) stimulated Na(+) uptake, whereas treatment with phenylephrine (an α(1)-adrenergic receptor agonist) as well as clonidine (an α(2)-adrenergic receptor agonist) significantly reduced Na(+) uptake, suggesting ...

Reovirus type 3 interfered with the binding of beta-adrenergic antagonist ligands to receptors on Y1 adrenal, C6 glioma, and mouse L cells. This inhibition of beta-adrenergic binding was dose related. Reovirus did not interfere with dopaminergic binding or isoproterenol-induced activation of adenylate cyclase. In addition, reovirus infected Y1 cells, which bind beta-adrenergic antagonist ligand...

Neurons located in the locus coeruleus project to hippocampus and provide noradrenergic innervation necessary for hippocampal-dependent learning and memory. The mechanisms underlying the function of norepinephrine (NE) in memory processing are unknown but likely reside in the ability of NE to modulate the efficacy of glutamate synaptic transmission via activation of G-protein-coupled adrenergic...