The development of molecular techniques, in particular the use of molecular markers have been used to identify markers linked to useful traits and made great advances in recent years. Amplified fragment length polymorphism (AFLP) analysis was used to analyze the geographical differentiation, phylogenetic relationships and to identify molecular markers linked to taro leaf blight resistance genes...

BACKGROUND AND AIMS Populations of oak (Quercus petraea and Q. robur) were investigated using morphological and molecular (AFLP) analyses to assess species distinction. The study aimed to describe species distinction in Irish oak populations and to situate this in a European context. METHODS Populations were sampled from across the range of the island of Ireland. Leaf morphological characters...

Amplified Fragment Length Polymorphism (AFLP) markers are formed by selective amplification of DNA fragments from digested total genomic DNA. The technique is popular because it is a relatively inexpensive way to produce large numbers of reproducible genetic markers. In this paper, we describe a Bayesian approach to modeling AFLP marker evolution by nucleotide substitution and an MCMC approach ...

Arbitrarily-primed DNA markers can be very useful for genetic fingerprinting and for facilitating positional cloning of genes. This class of technologies is particularly important for less studied species, for which genome sequence information is generally not known. The technologies include Randomly Amplified Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Amplified Fragmen...

The genetic diversity of a large number of pistachio genotypes grown in Iran is not exactly known. Most of the studies on genetic diversity of Iranian pistachio varieties are based on morphological characteristics or isozyme markers. In the present study, the genetic diversity of selected pistachio cultivars along with some wild species were evaluated by Amplified Fragment Length Polymorphism (...

three iranian native strains and three japanese commercial lines of the silkworm bombyx mori were analyzed using amplified fragment length polymorphism (aflp) markers. a set of ten psti/taqi primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. estimates of nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...

Seedless barberry (Berberis vulgaris L. var. asperma) is one of the few crops that is only cultivated in eastern parts of Iran. As a new crop there has not been any study to identify phylogenetic relationships of this plant with other related species existing in Iran. In this study, Amplification fragment length polymorphism (AFLP) markers based on four selected primer combinations (EcoRI/Tru1I...

Genetic diversity of fungal endophytes, Neotyphodium species, was studied in grasses Festuca arundinacea, F. pratensis and Lolium perenne using AFLP  markers. Fungi were isolated from the host leaf sheaths and Neotyphodium species were selected based on morphological characteristics. To confirm identity of selected fungi belonging to the genus Neotyphodium, polymerase chain reaction was perform...

Amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat markers were employed to characterize a genetic resource collection of Miscanthus, a grass under trial in Europe as a biomass crop. The 26 polymorphic markers produced by two ISSR fingerprinting primers were able to discriminate taxa and identify putative clones. AFLP fingerprints were fully reproducible and produced...

We report on the construction of sex-specific high-density linkage maps and identification of sex-linked markers for the black tiger shrimp (Penaeus monodon). Overall, we identified 44 male and 43 female linkage groups (2n = 88) from the analysis of 2,306 AFLP markers segregating in three full-sib families, covering 2,378 and 2,362 cM, respectively. Twenty-one putatively homologous linkage grou...