Herein, we have elaborated a new electrochemical aptasensor for the detection of ultra-trace amounts aflatoxin M1 (AFM1) present in cow milk, with regulated low levels. To achieve AFM1, simple strategy deposition specific aptamers was elaborated, based on their grafting pectin-reduced gold nanoparticles (AuNPs) electrogenerated graphene oxide sheet modified glassy carbon electrode. The elaborat...

Five million children aged less than five years die annually due to diarrhoea. The aim of the study was to identify some possible contributing factors for persistent diarrhoea. Seven weaning foods, including a locally-made food, were evaluated by estimating the microbial load using the most probable number method and aflatoxin levels (AFM1, AFG1, AFG2, and AFB2) by immunoaffinity column extract...

Aflatoxin M1 (AFM1) is a major metabolite of aflatoxin B1 (AFB1), which is formed when animals ingest feed contaminated with aflatoxin B1. The AFB1, once ingested by the animal, is rapidly absorbed by the gastrointestinal tract and is transformed into the metabolite AFM1, which appears in the blood after 15 minutes and is then secreted in the milk by the mammary gland (Van Egmond, 1989; Battaco...

OBJECTIVE To assess the risk of mycotoxin exposure (aflatoxin M1, ochratoxin A, and zearalenone) in celiac disease (CD) breastfeeding mothers and healthy control mothers, as well as in their offspring, by quantifying these contaminants in breast milk. STUDY DESIGN Thirty-five breastfeeding women with CD on a gluten-free diet and 30 healthy breastfeeding controls were recruited. Milk sampling ...

BACKGROUND The major contaminants usually encountered in milk and milk products include pesticide residues, heavy metals, and aflatoxin M1 (AFM1). Primarily, milk get contaminated before milching, from the cattle feed, from sources/materials used during the processing of milk as well as improper handling of the milk during the pre- and postprocessing period. OBJECTIVE The purpose of this stud...

The present study was aimed at measuring the concentration of aflatoxin M1 (AFM1) in milk Holstein Friesian cows, its effect on quality and seasonal trends, as well to investigate efficacy a commercial clay-based toxin binder. For this purpose, samples from dairy cows (n = 72) were collected assayed for AFM1 before employing selected animals, revealing that 69.4% had levels above United States ...

Background: Aflatoxin M1 (AFM1) is a toxic compound that could be found in milk and dairy products. AFM1 is the principle hydroxylated derivative of aflatoxin B1 (AFB1), formed in liver and excreted into the milk in the mammary glands of both human and lactating animals that have been fed with AFB1 contaminated diet. To the best of our knowledge, there is no report on stability of AFM1 during p...

aflatoxin m1 (afm1) is the hydroxylated metabolite of aflatoxin b1 (afb1) that it can be found in milk and dairy products. in this study, elisa (enzyme linked immunosorbent assay) technique was used for detection of afm1 in ice-cream in guilan province (northern iran). a total of 90 ice-cream samples was randomly obtained from different supermarkets. in 62 of the 90 ice-cream samples examined (...

aflatoxin m1 (afm1) is an important mycotoxin frequently found in milk and dairy products. dairy products and colostrum may be contaminated by aflatoxin m1 when dairy cattle have fed with aflatoxin b1-contaminated feeds. this study was undertaken in a dairy farm around tehran province to determine the presence and level of aflatoxin m1 (afm1) in spray dried and lyophilized colostrum samples. in...

Background and Aims: Cowchr('39')s milk is a daily staple food for many individuals that can be contaminated with many toxins such as aflatoxin M1 (AFM1). AFM1 is a chemical form of the aflatoxin B1 produced by some species of Aspergillus genus like A. ochraceus, A. flavus, A. nomius, and A. parasiticus that can contaminate feed and forage cattle. This toxin enters into the milk after eating co...