A total of 180 serum samples (107 cows, 73 buffaloes) from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT) and indirectELISA (i-ELISA). The overall prevalence of brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and 24.30% in cows 9.59%, 12.33% and ...

AIMS To evaluate the Pastorex aspergillus antigen latex agglutination test for the diagnosis of invasive aspergillosis in patients undergoing liver or bone marrow transplantation. METHODS Serum samples were taken at least twice weekly post-transplant and tested for Aspergillus antigen. Latex agglutination test results were compared with microbiological examination of respiratory, urine and bi...

Given the technical difficulties of the ELISA method, a gelatin particle agglutination test (MLPA) has been developed recently for the detection of anti-PGLI antibodies. The purpose of this study was to compare these 2 tests. MLPA was found to be less specific than ELISA (91% versus 98%, chi 2 = 66.8, p less than 0.001). The sensitivity of both tests was of 95% for the diagnosis of multibacilla...

Four hundred and six serum samples from buffalo (Syncerus caffer) were tested for leptospirosis, using the microscopic agglutination test. Seven buffaloes (1.7%) reacted positive and 27 (6.6%) inconclusive. Reactions against L. tarassovi and L. hardjo were the most prevalent.

Hen antigonococcal lipopolysaccharide hen serum was used in a simple slide agglutination test for the identification of Neisseria gonorrhoeae from primary isolates.

Of the methods now available for the detection of coagulase positive staphylococei, none is designed to show both clumping factor and free coagulase activity in a single system. The observations reported here are by-products of a search for variant strains of staphylococci with particular reference to strain differences in the cell surface component known as clumping factor or bound coagulase. ...

INTRODUCTION Leptospirosis is caused by a bacterium of the genus Leptospira. This study aimed at investigating the seroprevalence of and risk factors for leptospirosis in humans in Manaus, State of Amazonas. METHODS Interviews were performed, and 1,000 blood serum samples were examined using a microscopic agglutination test. RESULTS Forty-three cases were positive; there were 10 serotypes, ...

Objective. We quantitatively assessed the influence of oxidants on antigen-antibody-binding activity. Methods. We used several immunological detection methods, including precipitation reactions, agglutination reactions, and enzyme immunoassays, to determine antibody activity. The oxidation-reduction potential was measured in order to determine total serum antioxidant capacity. Results. Certain ...

We read the article by Dutta et al on “Leptospirosis An Overview” with interest.1 The problem in utilizing microscopic agglutination test (MAT) has been highlighted and we shall discuss briefly the current status of MAT. MAT is considered the gold standard test for diagnosis of leptospirosis. It has unsurpassed specificity, but its sensitivity is low compared to ELISA/SAT (Slide agglutination t...