During Pecorino Romano PDO cheese production, scotta (residual whey from ricotta manufacturing) or siero (whey) can be integrated with autochthonous starters, natural and composed of an indefinite number species strains, commercial selected starters to obtain scotta/siero-innesto. In this study, three biodiverse starter cultures (SR30, SR56, SR63) belonging the Agris Sardegna BNSS microbial col...

Accuracy in translation of the genetic code into proteins depends upon correct tRNA-mRNA recognition in the context of the ribosome. In human tRNA(Lys,3)UUU three modified bases are present in the anticodon stem-loop--2-methylthio-N6-threonylcarbamoyladenosine at position 37 (ms2t6A37), 5-methoxycarbonylmethyl-2-thiouridine at position 34 (mcm5s2U34) and pseudouridine (psi) at position 39--two ...

Introducción. La trazabilidad se considera en los sistemas empresariales como una herramienta de seguimiento y control enfocada la medición recolección datos para asignación eficiente recursos. El sector agrícola no es ajeno a esta práctica porque al igual que otros industriales, integra necesidades nivel cultivo, abastecimiento insumos, transformación, transporte comercialización productos. Ob...

Antisuppressor mutations reduce the efficiency of nonsense suppressors. A mutation in the gene sin4 of Schizosaccharomyces pombe leads to loss of 5-(methoxycarbonylmethyl) thiouridine (mcm5s2U) from the first anticodon position of tRNAs. This resembles the phenotype of sin3 (Heyer, W. D., Thuriaux, P., Kohli, J., Ebert, P., Kersten, H., Gehrke, C., Kuo, K. C., and Agris, P. F. (1984) J. Biol. C...

A combination of bioinformatic tools, high-throughput gene expression profiles, and the use of synthetic promoters is a powerful approach to discover and evaluate novel cis-sequences in response to specific stimuli. With Arabidopsis (Arabidopsis thaliana) microarray data annotated to the PathoPlant database, 732 different queries with a focus on fungal and oomycete pathogens were performed, lea...

BACKGROUND Hypericum perforatum L. (St. John's wort) is a medicinal plant with pharmacological properties that are antidepressant, anti-inflammatory, antiviral, anti-cancer, and antibacterial. Its major active metabolites are hypericins, hyperforins, and melatonin. However, little genetic information is available for this species, especially that concerning the biosynthetic pathways for active ...

Preand post-harvest physiology and quality responses of green pepper (Capsicum annuum L. cv ‘Standar p.13/0211003-01-Agris’) on exogenous Gibberellic acid-GA3 (100 μM), ProhexadioneCalcium (100 mg l), Cycocel (100 mg l) and Ethephon (100 mg l) applied as foliar sprays, were investigated. Among PGRs, GA3 @ 100μM was effective in promoting flowering and better for vegetative characteristics. Chlo...

Accuracy in translation of the genetic code into proteins depends upon correct tRNA–mRNA recognition in the context of the ribosome. In human tRNA UUU three modified bases are present in the anticodon stem–loop—2-methylthio-N6-threonylcarbamoyladenosine at position 37 (mstA37), 5-methoxycarbonylmethyl-2-thiouridine at position 34 (mcmsU34) and pseudouridine (c) at position 39—two of which, mstA...