In present study 7 carriers such as agar –agarose, wood fiber, wood chips, gelatin, wood powder, calcium alginate and butanol sodium alginate were used for immobilization of fungal amylase. Amylase immobilization was carried out by simple adsorption, adsorption followed by cross-linking, combined adsorption and gel entrapment methods. The suitability of the supports and techniques for the immob...

In addition to good mechanical properties needed for three-dimensional tissue engineering, the combination of alginate dialdehyde, gelatin and nano-scaled bioactive glass (45S5) is supposed to combine excellent cellular adhesion, proliferation and differentiation properties, good biocompatibility and predictable degradation rates. The goal of this study was to evaluate the in vitro and in vivo ...

The currently used approaches in the treatment of wounds and burns have been studied for many years to eliminate problems related with mechanical strength, elasticity, biocompatibility cost. Nowadays, fabrication composite fibers by a fiber as core hydrogels shell, which can be seeded cells is rapidly increasing. In this study, it aimed produce natural polymer-based dressing that provide contro...

Probiotic bacteria such as Lactobacillus rhamnosus GG (LGG) play an important role in maintaining a healthy microbiota environment. Oral administration is the only way to deliver probiotic small intestine. Due harsh environment of gastrointestinal tract, proportion can reach target site, limiting their beneficial effects. Although many encapsulation techniques exist, loss bacterial cell viabili...

727 Abstract: The gelatin cap-sule shell may be soft or hard depending on their formulation. Capsules are intended to be swallowed whole by the patient. In instances where patients (especially children) are unable to swallow capsules, the contents of the capsule can be removed and added (e.g., sprinkled) on soft food immediately before ingestion. In the manufacture of pharmaceuticals, encapsula...

BACKGROUND In this study, 293T cells were genetically engineered to secrete tissue inhibitor of metalloproteinase-2 (TIMP2) and encapsulated into alginate microcapsules to continuously release TIMP2 protein. METHODS The anti-invasive potential of the microcapsules was studied in vitro using brain tumor cells. The TIMP2 gene was transfected to 293T cells, and genetically engineered 293TIMP2 ce...

We report on the development of a new array-based screening flat platform with the potential to be used as a high-throughput device based on biomimetic polymeric substrates for combinatorial cell/3D biomaterials screening assays in the context of tissue engineering. Polystyrene was used to produce superhydrophobic surfaces based on the so-called lotus effect. Arrays of hydrophilic regions could...

Biomaterials with characteristics similar to extracellular matrix and suitable bioprinting properties are essential for vascular tissue engineering. In search biomaterials, this study investigated the three hydrogels alginate/hyaluronic acid/gelatin (Alg/HA/Gel), pre-crosslinked alginate di-aldehyde gelatin (ADA-GEL), methacryloyl (GelMA) respect their mechanical survival, migration, proliferat...