Objective(s) Articular cartilage tissue defects cannot be repaired by the proliferation of resident chondrocytes. Autologous chondrocyte transplantation (ACT) is a relatively new therapeutic approach to cover full thickness articular cartilage defects by in vitro grown chondrocytes from the joint of a patient. Therefore, we investigated the redifferentiation capability of human chondrocytes ma...

This paper reports a new approach for constructing blood vessel-like tissues using microfluidic devices made of calcium-containing agarose hydrogel. Sodium alginate (NaA) solutions with different cell types at high densities were introduced stepwise into the hydrogel microchannel, to deposit cell-containing multiple Ca-alginate hydrogel layers on the channel surface with the help of Ca 2+ ions ...

Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2hydroxy-2-phenylacetonitrile) to (R)-(-)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks and in a packed bed reactor. In shake flasks the ...

Myocardial infarction is caused by an interruption of coronary blood flow, leading to one the main death causes worldwide. Current therapeutic approaches are palliative and not able solve loss cardiac tissue. Cardiosphere derived cells (CDCs) reduce scarring, increase viable myocardium, with safety adequate biodistribution, but show a low rate engraftment survival after implantation. In order r...

Alginate is a commonly used biomedical hydrogel whose in vivo degradation behavior is only beginning to be understood. The use of alginate in the central nervous system is gaining popularity as an electrode coating, cell encapsulation matrix, and for duraplasty. However, it is necessary to understand how the hydrogel will behave in vivo to aid in the development of alginate for use as a neural ...

"Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cellbased sensor arrays" (2014). Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hy...

Chondrogenic differentiation of mesenchymal stromal cells (MSCs) in the form of pellet culture and encapsulation in alginate beads has been widely used as conventional model for in vitro chondrogenesis. However, comparative characterization between differentiation, hypertrophic markers, cell adhesion molecule and ultrastructural changes during alginate and pellet culture has not been described....

Citrus genus is known as one of the world's major economic agricultural products. In the current research, a method is reported for artificial seed production technology by shoot tips in Citrus sinensis.var. Thamson navel. Alginate is one of the most efficient gelling agents in the production of capsule coating in artificial seeding technique. In the current research, impact of different consti...

objective(s) articular cartilage tissue defects cannot be repaired by the proliferation of resident chondrocytes. autologous chondrocyte transplantation (act) is a relatively new therapeutic approach to cover full thickness articular cartilage defects by in vitro grown chondrocytes from the joint of a patient. therefore, we investigated the redifferentiation capability of human chondrocytes mai...

A method is described for the wet spinning of alginate fibers with a loading of single-walled carbon nanotubes as high as 23 wt%. Electrostatic assembling of polyelectrolytes and nanotubes coated with sodium dodecyl sulfate is exploited by using calcium as a cross-linking agent. The Young’s modulus of these fibers depends non-monotonically on nanotube concentration which is explained using Halp...