The ultrastructural localization of H202 production in suspended polymorphonuclear leukocytes (PMN) stimulated with particles was studied using CeCI3 technique. PMN stimulated with opsonized zymosan or polystylene latex with or without lgG were incubated in 0.1 M Tris-maleate buffer with 1 mM CeCI, and 10 mM aminotriazole. Cells were then fixed and embedded in a resin for electron microscopy. T...

Bodies with the morphologic and cytochemical characteristics of peroxisomes have been identified in the satellite and Schwann cells of rat dorsal root ganglia. They are membrane-delimited, round or oval structures which contain a moderately electron-dense matrix but lack a crystalline core. On incubation of the tissue in a cytochemical medium for demonstration of peroxisomes, these bodies show ...

Adriamycin and daunomycin produce glutathione, by ‘4C-formate oxidation, dose-related cardiac toxicity that may be and by the catalase-aminotriazole trapping related to oxygen radicals. Addition of technique. These experiments indicate these compounds to human erythrocyte that Adriamycin and daunomycin interact suspensions resulted in stimulation of with oxyhemoglobin to generate reactive hexos...

The activities of the endogenous O2- and H2O2 scavenging enzymes, superoxide dismutase (SOD), glutathionine peroxidase (GP), and catalase, were measured in lysates of the intracellular parasite, Toxoplasma gondii, and in various macrophage populations. During 72 h of cultivation in standard medium alone, the catalase activity of in vivo-activated toxoplasma-immune macrophages (IM) and immune-bo...

We have investigated what limits demand-driven de novo glutathione (GSH) biosynthesis in green Arabidopsis suspension culture cells. GSH is the most abundant low-molecular weight thiol in most plants and can be quantified using monochlorobimane to fluorescently label GSH in live cells. Progress curves for labeling reached a plateau as all the cytoplasmic GSH was conjugated. In the presence of e...

Extracellular and intracellular reactive oxygen species attack different targets and may, therefore, result in different forms of oxidative stress. To specifically study an oxidative stress induced by a regulated intracellular flux of a defined reactive oxygen species in endothelium, we used immunotargeting of the H2O2-generating enzyme glucose oxidase (GOX) conjugated with an antibody to plate...