Human amniotic membrane is a standard substratum used to culture limbal epithelial stem cells for transplantation to patients with limbal stem cell deficiency. Various methods were developed to decellularize amniotic membrane, because denuded membrane is poorly immunogenic and better supports repopulation by dissociated limbal epithelial cells. Amniotic membrane denuding usually involves treatm...

The protective amniotic sac that is covering the foetus plays a very pivotal role in many aspects .The material which was once thought to be discarded during the delivery of the baby has now found immense applications for regeneration and revitalization of devitalized tissues. The amniotic membrane can be processed and the dehydrated amnion allograft can be used for ocular transplants, treatmen...

PURPOSE To reexamine whether the alpha5 chain of type IV (alpha5[IV]) collagen, thought to be absent, is in fact present in human amniotic membrane. METHODS Cryosections of human amniotic membrane obtained at Cesarean section were immunohistochemically examined for the presence of alpha5(IV), with or without inclusion of the denaturing step. Amniotic membrane was digested with collagenase to ...

BACKGROUND Standard compression therapy for venous ulcers of the legs does not promote healing. Although autografting accelerates tissue repair, it is difficult to use in patients with concomitant diseases or when multiple grafts are required. The amniotic membrane has been used as a covering material and promotes epithelialization, making it a good potential treatment option when autografts ar...

BACKGROUND Oronasal fistulas are a frequent complication after cleft palate surgery. Numerous repair methods have been described, but wound-healing problems occur often. The authors investigated, for the first time, the suitability of multilayered amniotic membrane allograft for fistula repair in a laboratory experiment (part A), a swine model (part B), and an initial patient series (part C). ...

The first reported use of fetal membrane as skin substitute was by Davis in 1910. 1 In 1913, Salbella presented the first clinical report of successful use of amniotic membrane in the treatment of burns and skin ulcerations. 2 In 1940, DeRoth reported the use of amniotic membrane in the repair of conjuctival defects. From 1940 to 1965 a number of clinical trials of successful use of amniotic me...

1. Letko E, Stechschulte SU, Kenyon KR, et al. Amniotic membrane inlay and overlay grafting for corneal epithelial defects and stromal ulcers. Arch Ophthalmol. 2001;119:659-663. 2. Ferreira De Souza R, Hofmann-Rummelt C, Kruse FE, Seitz B. Multilayer amniotic membrane transplantation for corneal ulcers not treatable by conventional therapy—a prospective study of the status of cornea and graft d...

 Amniotic membrane has been employed in the treatment of wounds for almost 100 years, beginning with early application of natural amniotic membrane obtained from labor and delivery to various types of burns and wounds. Amniotic membrane is rich in collagen and various growth factors that support the healing process to both improve wound closure and reduce scar formation. Unique properties...