The Micro-Media Systems (MMS) anaerobe susceptibility testing panel results from four laboratories were compared for interlaboratory and intralaboratory variations and for the results with the reference agar dilution and a broth microdilution method. The interlaboratory agreement was 98.0% and intralaboratory agreement was 97.3% (+/- 1 log2 dilution). When interpretive criteria for each antimic...

A compartmented, autoclavable culture vessel has been developed for the purpose of studying interactive associations of microorganisms which are essential to the anaerobic decomposition of sewage sludge. The unit employs sterile filter membranes to subdivide the interior culture space into individual compartments. Bacteria cultured in one compartment are denied access to adjacent compartments, ...

In this work, corrections are made to the descriptions of the species Anoxybacillus pushchinoensis corrig. and the genus ANOXYBACILLUS: Experiments to determine the relationship of A. pushchinoensis K1(T) to oxygen showed that it was capable of aerobic growth, but preferred to grow anaerobically. During aerobic growth, the redox indicator resazurin was reduced as a result of hydrogen gas produc...

Necrotizing fasciitis is characterized by a rapidly progressing necrosis of subcutaneous fat and fascia that rarely occurs in head and neck. It can be life threatening in the case of late diagnosis and antibiotic therapy and without surgical intervention. It is caused by group A streptococci or by a synergistic combination of aerobe and anaerobe microorganisms and bacterial synergy is an import...

In the filtrates of typhus-infected tissues of guinea pigs can be occasionally found a substance which produces in these animals thermic reactions, lesions characteristic of experimental typhus, and, still less frequently, inimunity to later injections of active virus. The general indications are that this substance is not a living organism.

The effects of MgADP and MgATP on the kinetics of a pre-steady-state electron-transfer reaction and on the steady-state kinetics of H2 evolution for nitrogenase proteins of K. pneumoniae were studied. MgADP was a competitive inhibitor ofMgATP in the MgATPinduced electron transfer from the Fe-protein to the Mo-Fe-protein. A dissociation constant K' = 20,UM was determined for MgADP. The release o...

BackgroundSurgical site infections are a global patient safety concern. Due to lack of evidence on contamination, pre-set surgical goods sometimes disposed or re-sterilized, thus increasing costs, resource use, and environmental effects.AimTo investigate time-dependent bacterial air contamination covered uncovered sterile in the operating room.MethodsBlood agar plates (N = 1584) were used detec...

Today an optimization of workout units and the competitions is more important than ever. That applies especially for amateur athletes, who practice beside their normal work. An optimization with the modern lactate analysis is too expensive and not practical for this group of athletes. In this paper we will present a model based alternative, called PerPot. By means of simulation, workout units a...

The bacillus described in this paper occurred in a fatal case of gas gangrene. The cultures were derived from infected muscle at a considerable distance from the wound surface, and no other anaerobes were present in the muscle. The bacillus proved markedly pathogenic for guinea pigs, producing extensive muscle lesions from which pure cultures of the bacillus were always obtained. Thus there is ...