The HindII + III restriction enzyme fragmentation pattern of various lambda-phi80trp deoxyribonucleic acid molecules is presented. An analysis of deoxyribonucleic acid molecules carrying deletions ending within the trp regulatory elements and a deoxyribonucleic acid molecule carrying a deletion within trpE indicates that a fragment of 8.3 X 10(5) daltons contains at least part of the trp promot...

Rauscher leukemia virus deoxyribonucleic acid polymerase is reversibly inactivated by 6 m guanidine-hydrochloride. Gel filtration in 6 m guanidine-hydrochloride reveals that the viral deoxyribonucleic acid polymerase consists of a single polypeptide chain of approximately 70,000 molecular weight.

The endonuclease of Bacillus subtilis specific for single-stranded deoxyribonucleic acid is absent in spores, appears during germination only after the start of deoxyribonucleic acid synthesis, and is located almost exclusively in the periplasm.

Preparations of nucleic acids obtained by extraction of mouse liver, HeLa cells and cell fractions with phenol and deoxycholate have been characterized with regard to the differential solubility of ribonucleic acid and deoxyribonucleic acid in ethanol, density-gradient centrifugation and the presence of high-molecular-weight contaminants. Ribonucleic acid obtained by this method is less soluble...

hepcidin is an innate immune element which decreases the iron absorption from diet and iron releasing from macrophage cell. in contrast to the chemical iron chelators, there has been limited effort applied to the specific use of hepcidin as a new drug for decreasing the iron overload. hepcidin is produced in different biological systems. for instance, e-coli is used for human hepcidin expressio...

The enzyme deoxyribonucleic acid polymerase, purified from Escherichia coli, catalyzes extensive formation of deoxyribonucleic acid by a mechanism that involves replication of a deoxyribonucleic acid primer. This replication requires the presence of the four deoxynucleoside triphosphates commonly found in deoxyribonucleic acid, and deoxyribonucleic acid itself (1). In the absence of one, two, o...

Plasmid-to-chromosome ratios in Enterobacteriaceae, upon interruption of protein synthesis by chloramphenicol, are either conserved or increased when measured by dye buoyant density centrifugation. We have found, on the other hand, that the effect of inhibition of protein synthesis on the amount of covalently closed circular deoxyribonucleic acid visualized by this method in two strains of Stre...

The following compounds, which possess anti-R-plasmid activity (Hahn and Ciak, 1976) in decreasing order, were shown by viscometric titration to change systematically the superhelical density of closed circular PM2 deoxyribonucleic acid in the manner of intercalators: ethidium bromide, quinacrine, acridine orange, quinine, chlorpromazine, chloroquine, and methylene blue. The same effect was cau...

Enhancement of pigment production potential Serratia marcescens (GBB151) through mutation and random amplified polymorphic deoxyribonucleic acid analysis its mutantsCecilia Nireti Fakorede, Babamotemi Oluwasola Itakorode, Olu Odeyemi, Gbolahan Babalola