introduction: chlamydia is an important cause of sexually transmitted diseases leading to tubal factor infertility. background: this study aims to define the role of chlamydial antibody detection in predicting presence, nature and type of tubal pathology in laparoscopy. materials and methods: a prospective study was conducted on 200 consecutive patients undergoing laparoscopy as a part of infer...

A novel lateral flow immunoassay biosensor, combining the receptor-ligand reaction and antigen-antibody was developed for detection of ?-lactam antibiotics in milk. The receptor protein, carboxy-terminal domain sensor-transducer mutant from Bacillus. licheniformis, served as recognition element. Colloidal gold (CG)-labelled antibody-receptor ternary complex applied a signal transducer probe. pr...

conclusions based on the findings, application of recombinant arcaga antigen for the detection of h. pylori infection is a simple, rapid, and brief non-invasive method. therefore, it can be suggested as an appropriate antigen for h. pylori detection kits. results we investigated caga protein, using the immunoblotting method in stool samples, which were already identified as positive by the appl...

background: cholera, an infectious disease caused by vibrio cholerae , is primarily transmitted by ingestion of contaminated food or water. in severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death.   methods: in this study v.cholerae non-o1 was cultured in suitable media. lps was extracted from the surface of  bacteria by hot pheno...

background: the aim of this study was using elisa for detection of anti- schistosoma haemato­bium antibodies in both sera and urine of patients with urinary schistosomiasis. methods: thirty three sera and urine samples were collected from patients with acute schisto­somi­asis in diyala province, east of iraq in 2006.   their diseases were confirmed by find­ing   s. haematobium ova in urine exam...

systemic sclerosis is a generalized disorder of connective tissue clinically characterized by thickening and fibrosis of the skin and by distinctive forms of involvement of internal organs. one of the hallmarks of systemic sclerosis is the presence of serum autoantibodies against a variety of nuclear and cytoplasmic antigens. the primary purpose of this study was to identify the autoantibodies ...

Aqueous glutaraldehyde has been polymerized under alkaline conditions in the presence of a surfactant to yield microspheres of varied diameters. Microbeads of a nominal 300 nm diameter, made fluorescent via fluoresceinisothiocyanate, were used as a carrier of rabbit anti-human red blood cell antibodies. Specific labeling of the human red blood cells as well as diagnostic use of these fluor...

Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...

turnip curly top virus (tctv) is a member of the newly established genus turncurtovirus, family geminiviridae. development of an efficient detection system is necessary to study the biological characteristics of viruses. due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of t...