A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensiti...

Immunization of rabbits with purified catechol methyltransferase from Candida tropicalis yielded a potent antiserum. Ouchterlony double diffusion analysis showed a single precipitin line. There was no cross reactivity with the catechol methyltransferase from rat and bovine liver. Specific antigen-antibody interaction was demonstrated by a potent inhibitory effect of the antibody on the yeast en...

The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of st...

A microneutralization test in PK(15) cells was developed to measure the neutralizing antibody response of a group of ponies experimentally challenged with louping ill virus. Viral cytopathic effect was maximal after 6 days of incubation, at which point titration endpoints were clear-cut and readily determinable. The assay compared favorably with the mouse neutralization test for accuracy and ea...

Background & Aims: Hepatitis B virus is one of the causes of acute and chronic hepatitis. Due to multiple hospitalization and blood transfusion, thalassaemic patients are at higher risk of this infection. Duration of the immunity after vaccination in this high-risk group and the need for booster vaccination is uncertain. The aim of this study was to find antibody titration and immunity to Hepat...

The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, d...