Antigenic divergence has been found between Bordetella pertussis vaccine strains and circulating strains in several countries. In the present study, we analyzed B. pertussis isolates collected in Japan from 1988 to 2001 using pulsed-field gel electrophoresis (PFGE) and sequencing of two virulence-associated proteins. The 107 isolates were classified into three major groups by PFGE analysis; 87 ...

objective(s): helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. the vacuolating cytotoxin (vaca) is an effective virulence factor involved in gastric injury. the aim of this study was to construct a recombinant protein containing antigenic region of vaca gene and determine its antigenicity.   materials and methods: the antigenic region of v...

The demonstrated link between the emergence of H3N2 variant (H3N2v) influenza A viruses (IAVs) and swine exposure at agricultural fairs has raised concerns about the human health risk posed by IAV-infected swine. Understanding the antigenic profiles of IAVs circulating in pigs at agricultural fairs is critical to developing effective prevention and control strategies. Here, 68 H3N2 IAV isolates...

ERAP1 (endoplasmic reticulum aminopeptidase 1), ERAP2 and IRAP (insulin-regulated aminopeptidase) are three homologous enzymes that play critical roles in the generation of antigenic peptides. These aminopeptidases excise amino acids from N-terminally extended precursors of antigenic peptides in order to generate the correct length epitopes for binding on to MHC class I molecules. The specifici...

conclusions results indicates that antigenic region of recombinant caga protein were recognized as an antigen , so it might be a candidate for the development of h. pylori vaccine, elisa kit designs and serological diagnosis of h. pylori infections. background helicobacter pylori is a human pathogen that causes chronic gastritis, which playsrole in gastric and duodenal ulcers, is also involved ...

Western blot analysis was used to identify antigenic components of Gardnerella vaginalis. Polypeptides bound to nitrocellulose membranes were probed with murine antisera raised to two strains of G. vaginalis, and antibody-antigen complexes were detected with 125I-labelled antimouse immunoglobulin followed by autoradiography. Although there was inter-strain variation in immunogenic polypeptide p...

9:00 Welcome Annemiek Geluk LUMC ‘Development of novel T cell assays for detection of M. leprae infection’ Linda Oskam, KIT, The Netherlands ‘New and improved opportunities for serological KIT, testing in leprosy’ John Spencer, CSU, USA ‘Use of Western blot, ELISA and protein microarrays to define disease-state-specific antigenic profiles in leprosy patients’ Sang-Nae Cho, YUMC, Korea ‘Enzyme i...

Protein and antigenic heterogeneity among isolates of Bacillus piliformis, the etiologic agent of Tyzzer's disease, were investigated. The seven isolates utilized in this study were originally isolated from naturally infected animals of different animal species and diverse geographical locations. Isolates were propagated in mammalian cell lines, and bacterial extracts were prepared. Protein and...

Structures of BG505 SOSIP.664 trimer in complex with broadly neutralizing antibodies (bNAbs) have revealed the critical role of trimeric context for immune recognition of HIV-1. Presentation of trimeric HIV-1 antigens on nanoparticles may thus provide promising vaccine candidates. Here we report the rational design, structural analysis and antigenic evaluation of HIV-1 trimer-presenting nanopar...

A variety of methods are currently employed in attempts to identify antigenic determinants and other features of proteins. Most of these involve calculations based on scales of values for the 20 amino acids that reflect their likelihood of occurrence at the surface of proteins or as part of secondary structures such as helices or beta-bends. The most successful of these procedures all use scale...