Of 150 coagulase-positive staphylococcal isolates from infections in dogs, 9 isolates with biochemical properties of human biotype were characteristically agglutinated by absorbed antiserum to staphylococcal strain 17, while only 110 of 141 isolates with biochemical properties of staphylococci of canine origin were agglutinated by absorbed antiserum to strain 61218. However, upon development of...

The role of cell interactions in lymphocyte stimulation was analyzed by studying the kinetics of lymphocyte proliferation at different cell concentrations, and also by a lymphocyte microculture technique in solid medium. An absolute requirement for cell interactions was found in lymphocyte responses to concanavalin A, pokeweed mitogen, sodium periodate, purified protein derivative from Mycobact...

The neutralizing activities of fowl, rabbit and mouse antisera prepared against TRIC agents were enhanced, in some cases over a 100-fold, by the addition of antiserum against the appropriate IgG. Significant non-specific inactivation of the organisms was observed in the absence of antiserum against TRIC agents, in reaction mixtures containing normal serum and antiserum against IgG. Since the ex...

The present study describes a method for the production of a specific anti-T-cell receptor antiserum, and characteristics of its ability to block specific cell-mediated cytotoxicity in vitro. Immunization and antiserum adsorption procedures were designed to select for idiotypic differences in the recognition units of C3H lymphocytes immune to two different strains of mouse cells, such that the ...

Pemphigus is a human disease that causes extensive blistering of the skin. This blistering is related to a loss of epidermal cell cohesion and is accompanied by circulating autoantibodies that stain epidermal cell surfaces, as shown by immunofluorescence microscopy. One of the major components involved in epidermal cell cohesion is the desmosome. The pathological changes that accompany pemphigu...

BACKGROUND Mouse embryonic stem (ES) cells are derived from the inner cell mass (ICM) of the preimplantation blastocysts. So it is suggested that ES and ICM cells should have similar cellular surface molecules and antiserum to ES cells can inhibit ICM development. OBJECTIVE The objective of this study was to evaluate the effect of rabbit antiserum to ES cells on mouse preimplantation embryo d...

Antibodies were raised in rabbits against the purified voltage-dependent sodium channel from rat skeletal muscle sarcolemma. The resultant antiserum reacted with the purified channel in a solid-phase radioimmunoassay and precipitated the sodium channel from a crude mixture of solubilized membrane proteins. Crude membrane proteins separated according to size under nondenaturing conditions by chr...

Antiserum to mannophosphoinositides had a significant inhibitory effect on phospholipid synthesis in Mycobacterium smegmatis ATCC 607 at different phases of growth; however, the inhibition was more pronounced in the exponential phase. The rate of synthesis of total phospholipids and individual phospholipids, viz., cardiolipin, phosphatidylethanolamine, and mannophosphoinositides, decreased in t...