We used three heterogeneous parental cultures of LSC-AXC rat prostate cancer cells: LSC-AXC-C/0, cells maintained on culture medium; LSC-AXC-D/0, cells maintained on culture me dium containing 10~7 M 5<*-dihydrotestosterone; and LSC-AXCT/0, cells maintained on culture medium containing 10~7 M tes tosterone, to isolate clonally derived cell lines. Eleven of 15 clonal cell lines were tumorigenic ...

Growth and calcification of the marine coccolithophorid Emiliania huxleyi is affected by ocean acidification and macronutrients limitation and its response varies between strains. Here we investigated the physiological performance of a highly calcified E. huxleyi strain, NZEH, in a multiparametric experiment. Cells were exposed to different CO2 levels (ranging from 250 to 1314 μatm) under three...

Phosphorus (P) plays an important role in eutrophication and algal adaptation to environmental stresses; therefore, a better understanding of the impact P is essential control cyanobacterial bloom. In this study, Microcystis aeruginosa treated with 5 h ambient irradiation culture medium different availabilities dissolved organic (DOP) matter (DOM) explore physiological responses. Compared photo...

Alkaline phosphatases (APases) are important enzymes in organophosphate utilization. Three prokaryotic APase gene families, PhoA, PhoX, and PhoD, are known; however, their functional characterization in cyanobacteria largely remains to be clarified. In this study, we cloned the phoD gene from a halotolerant cyanobacterium, Aphanothece halophytica (phoD(Ap)). The deduced protein, PhoD(Ap), conta...

Abstract Key message Short-term nitrogen (N) deposition stimulates phosphorus (P) demand owing to the growth improvment of Phyllostachys edulis seedlings. Increased N loads led acquisition and utilization sufficient P, while limitation P starvation could be alleviated by higher activity soil acid phosphatase use efficiency rather than resorption from senescent organs. Context Plants in most ter...

Phosphorus (P) accumulators used for phytoremediation vary in their potential to acquire P from different high P regimes. Growth and P accumulation in Polygonum hydropiper were both dependent on an increasing level of IHP (1-8 mM P) and on a prolonged growth period (3-9 weeks), and those of the mining ecotype (ME) were higher than the non-mining ecotype (NME). Biomass increments in root, stem, ...

Phosphate (Pi) deficiency in soil system is a limiting factor for rice growth and yield. Majority of the soil phosphorus (P) is organic in nature, not readily available for root uptake. Low Pi-inducible purple acid phosphatases (PAPs) are hypothesized to enhance the availability of Pi in soil and cellular system. However, information on molecular and physiological roles of rice PAPs is very lim...

Marine microbes are adapted to surviving in a variable phosphorus (P) environment. This adaptation frequently involves the presence of periplasmic or cell membrane-associated enzymes that enable them access to alternative sources of P when phosphate is depleted. In a recent study we identified the phosphatase PhoX as an enzyme that may be essential in mediating organic P acquisition in the ocea...

Many arable lands have accumulated large reserves of residual phosphorus (P) and a relatively large proportion of soil P is less available for uptake by plants. Root released organic anions are widely documented as a key physiological strategy to enhance P availability, while limited information has been generated on the contribution of rhizosphere organic anions to P utilization by crops grown...