In this paper, we compare four field-of-view (FOV) metrics that, when applied to a low-resolution image of a microscope slide, are capable of both accurately delineating the specimen and selecting a subset of focus candidate FOVs required for construction of high-resolution focus map. The metrics evaluated are: threshold index (TI) that measures image intensity; normalised auto-correlation inde...

Development of analytical methods for the determination of phytosterols in whole grains is one of growing interests due to their clinical and nutritional functions in human. In this study, we examined simultaneous determination of β-sitosterol, stigmasterol and campesterol in Korean rice cultivars by liquid chromatography atmospheric chemical ionization time-of-flight mass spectrometer (LC-APCI...

We have already shown that the in-vacuum gas-phase Meerwein reaction of (thio)acylium ions is general in nature and useful for class-selective screening of cyclic (thio)epoxides. Herein we report that this gas-phase reaction can also be performed efficiently at atmospheric pressure under both electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) conditions. This alte...

Carotenoids and carotenoid esters were extracted from red pepper pods (Capsicum annuum L.) without saponification. Among the 42 compounds detected, 4 non-esterified, 11 mono- and 17 diesters were characterized based on their retention times, UV/Vis spectra and their fragmentation patterns in collision-induced dissociation experiments in atmospheric pressure chemical ionization mass spectrometry...

Oxidation products from triolein under model heated frying conditions have been analyzed using liquid chromatography with an evaporative light scattering detector and atmospheric pressure chemical ionization (APCI) mass spectrometric detection. Triolein was heated at 190 degrees C with 2% water added each hour, to simulate the moisture of a frozen product, until polar components reached approxi...

The coupling of liquid chromatography and mass spectrometry (LC/MS) has been established as one of the most powerful tools in analytical chemistry and has resulted in important advances especially in biomedical and biochemical research. The predominantly used interfaces are electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). Non-polar compounds, however, are diffi...

The protonation site of para-dimethylaminobenzoic acid (p-DMABA) was investigated using atmospheric pressure ionization methods (ESI and APCI) coupled with collision-induced dissociation (CID), nuclear magnetic resonance (NMR), and computational chemistry. Theoretical calculations and NMR experiments indicate that the dimethyl amino group is the preferred site of protonation both in the gas pha...

Silver-ion high-performance liquid chromatography (HPLC) coupled to atmospheric pressure chemical ionization mass spectrometry (APCI-MS) is used for the regioisomeric analysis of triacylglycerols (TGs). Standard mixtures of TG regioisomers are prepared by the randomization reaction from 8 mono-acid TG standards (tripalmitin, tristearin, triarachidin, triolein, trielaidin, trilinolein, trilinole...

Hyphenated HPTLC has been used to analyze several neutral sphingolipids acting as lysosomal storage disease (LSD) biomarkers. Automated multiple development (AMD) provides separation of lipid peaks, which are detected and quantified using fluorescence detection by intensity changes (FDIC) after primuline post-impregnation. A final online transfer to a mass spectrometer by means of an elution-ba...