The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquidliquid extraction of proteins. We investigated the solubilization of cytochrorne-c and 0-chymotrypsin into the NaDEHP reverse micellar phase by varying the pH and NaCl concentration in the aqueous phase. At neutral pH and relatively low ionic strength, the proteins are extracted into th...

Compound-specific carbon isotope ratio analysis is a promising tool to assess the origin and fate of organic contaminants in groundwater. The aim of this study was to develop and evaluate a reliable, fast method to determine carbon isotope ratios of chlorinated methanes, ethanes, and ethenes in aqueous samples. Direct solid-phase microextraction (dSPME) and headspace solid-phase microextraction...

PURPOSE Corneal endothelium in vivo is arrested in G1, the phase of the cell cycle that prepares cells for DNA synthesis. In many cell types, transforming factor (TGF)-beta inhibits proliferation by inducing G1-phase arrest. Evidence indicates that corneal endothelial cells synthesize mRNA for TGF-beta1 and are also bathed in aqueous humor that contains TGF-beta2 (mainly in a latent form). As s...

Genetically constructed microbial biosensors for measuring organic pollutants are mostly applied in aqueous samples. Unfortunately, the detection limit of most biosensors is insufficient to detect pollutants at low but environmentally relevant concentrations. However, organic pollutants with low levels of water solubility often have significant gas-water partitioning coefficients, which in prin...

the extraction-separation of lanthanum and europium ions with bis(2-ethylhexyl) phosphoric acid (dehpa) in cyclohexane was improved by adding 12-crown-4 (12c4) to the aqueous phase as a masking agent. in the presence of this crown ether the extraction curve of lanthanum ions versus ph shifts to the higher ph region, while the curve is not influenced for the europium ions. consequently the separ...

this study focuses on the preparation and modification of a thin-film composite (tfc) hollow fiber polyamide membrane fabricated by the interfacial polymerization of piperazine (pip) and trimesoyl chloride (tmc) on a porous polysulfone substrate. the effects of triethylenetetramine (teta) and silica nanoparticles (sio2) contents in the aqueous phase (as the additives) on the morphology and perf...

Objective(s):Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile.   Materials and Methods: In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters ...

Aqueous two-phase systems contained within microdroplets enable a bottom-up approach to mimicking the dynamic microcompartmentation of biomaterial that naturally occurs within the cytoplasm of cells. Here, we demonstrate the generation of femtolitre aqueous two-phase droplets within a microfluidic oil channel. Gated pressure pulses were used to generate individual, stationary two-phase microdro...