Analyzing extracellular vesicles (EVs) is an attractive approach to diagnosis of prostate diagnosis. However, existing methods of EVs isolation have low efficiency, purity, and long process time, and therefore have low diagnostic ability. To solve these the problems, a two-phase system is adapted to isolate EVs from a patient's urine. Urine from 20 prostate cancer (PCA) patients and 10 benign p...

The partitioning behavior of immunoglobulin G (IgG) in the aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and phosphate was studied. The parameters of ATPS exhibiting the pronounced effects on the partitioning behavior of IgG include phase composition, PEG molecular weight, and the addition of sodium chloride (NaCl). The accumulation of IgG at the interface of the ATPS ...

The intracellular environment in which biological reactions occur is crowded with macromolecules and subdivided into microenvironments that differ in both physical properties and chemical composition. The work described here combines experimental and computational model systems to help understand the consequences of this heterogeneous reaction media on the outcome of coupled enzyme reactions. O...

The current study explores the possibility of using a polyethyleneglycol(PEG)-ammonium sulphate aqueous two-phase system (ATPS) as an early step in a process for the purification of a model 6.1 kbp plasmid DNA (pDNA) vector. Neutralised alkaline lysates were fed directly to ATPS. Conditions were selected to direct pDNA towards the salt-rich bottom phase, so that this stream could be subsequentl...

Ethyl lactate is a hydrophilic solvent produced from bio-renewable sources (bioethanol and lactic acid corn fermentation) that considered as “green” due to its extremely low toxicity, biodegradability negligible eco-toxicity. This work focuses on the utilization of ethyl form aqueous two-phase systems (ATPS) in presence inorganic salts for extraction antibiotics solutions. The performance three...

Viscoelastic Networks In article number 2205649, Ho Cheung Shum and co-workers develop a new aqueous two-phase system (ATPS) that displays dynamic assembly of viscoelastic networks by liquid–liquid phase separation liquid–solid (AqLL-LS PS2). are generated using phase-separated DEX droplets as templates the subsequent liquid-to-solid transition PNIPAM-rich phase.

Surface properties of Streptomyces griseus cells, such as surface net charge and surface net hydrophobicity, were characterized by using the aqueous two-phase system (ATPS) method. It was found that the surface net charge of S. griseus cells was reduced after the POPC liposome-treatment of the cells at 41C. The surface net hydrophobicities of S. griseus cells and liposome-treated cells cultivat...

Scott Long et al. have constructed synthetic cells comprised of lipid bilayer membranes surrounding a two-phase, aqueous polymer solution that can be reversibly converted to a single phase. An understanding of compartmentalization in cell function has been hampered by the lack of an experimental model system. Using a previously developed method, the authors constructed artificial cells by encap...