BACKGROUND Arsenic as an environmental toxicant is able to exert malformations in male reproductive system by inducing oxidative stress. Vitamin E (Vit.E) is known as antioxidant vitamin. OBJECTIVE The aim of this study was to investigate the harmful effects of sodium arsenite on sperm parameters and the antioxidant effects of Vit.E on sperm anomalies in sodium arsenite treated rats. MATERI...

Environmental arsenic is a world-wide health issue, making it imperative for us to understand mechanisms of metalloid uptake and detoxification. The predominant intracellular form is the highly mephitic arsenite, which is detoxified by removal from cytosol. What prevents arsenite toxicity as it diffuses through cytosol to efflux systems? Although intracellular copper is regulated by metallochap...

The purification and initial characterization of arsenite oxidase from Alcaligenes faecalis are described. The enzyme consists of a monomer of 85 kDa containing one molybdenum, five or six irons, and inorganic sulfide. In the presence of denaturants arsenite oxidase releases a fluorescent material with spectral properties identical to the pterin cofactor released by the hydroxylase class of mol...

INTRODUCTION Ceratophyllum demersum L. is a widespread submerged macrophyte in aquatic environments. METHODS Simulation experiments were conducted in the laboratory to investigate arsenic (As) accumulation, speciation, and efflux of C. demersum exposed to arsenate and arsenite solutions. RESULTS Plant shoots showed a significant accumulation of As with a maximum of 862 and 963 μg As g(-1) d...

BACKGROUND Cells from patients with t(15;17) acute promyelocytic leukemia (APL) express the fusion protein between the promyelocytic leukemia protein and retinoic acid receptor alpha (PML/RAR alpha). Patients with APL respond to differentiation therapy with all-trans-retinoic acid, which induces PML/RAR alpha degradation. When resistance to all-trans-retinoic acid develops, an effective treatme...

Arima, Kei (University of Tokyo, Tokyo, Japan), and Michiko Beppu. Induction and mechanisms of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:143-150. 1964.-Pseudomonas pseudomallei strain 54, able to grow in the presence of 2 x 10(-2)m arsenite, was isolated from soil. After a short lag period, it grew at a normal growth rate. In the organisms grown with 10(-2)m arsenite, ox...

Our laboratory has shown that arsenite markedly increased the cancer rate caused by solar-simulation ultraviolet radiation (UVR) in the hairless mouse skin model. In the present study, we investigated how arsenite affected DNA photodamage repair and apoptosis after solar-simulation UVR in the mouse keratinocyte cell line 291.03C. The keratinocytes were treated with different concentrations of s...

Article history: Received on: 26/05/2014 Revised on: 02/07/2014 Accepted on: 24/08/2014 Available online: 27/08/2014 The present study was aimed to evaluate Vitamin C (VC) as a potent natural antioxidant to reduce the genotoxic effects of sodium arsenite in human lymphocytes. The study was design to assess the role of VC (10 μg/ml), upon sodium arsenite induced in vitro genotoxicity by using al...

In transgenic zebrafish (Danio rerio), green fluorescent protein (GFP) is a promising marker for environmental pollutants. In using GFP, one of the obstacles which we faced was how to compare toxicity among different toxicants or among a specific toxicant in different model species with the intensity of GFP expression. Using a fluorescence detection method, we first validated our method for est...

Exposure to arsenic in its inorganic form, arsenite, causes adverse effects to many different organs and tissues. Here, we have investigated arsenite-induced adverse effects on vascular tissues in the model organism zebrafish, Danio rerio. Zebrafish embryos were exposed to arsenite at different exposure windows and the susceptibility to vascular tissue damage was recorded at 72hours post fertil...