Endoplasmic reticulum (ER) thiol oxidases initiate a disulfide relay to oxidatively fold secreted proteins. We found that combined loss-of-function mutations in genes encoding the ER thiol oxidases ERO1α, ERO1β, and PRDX4 compromised the extracellular matrix in mice and interfered with the intracellular maturation of procollagen. These severe abnormalities were associated with an unexpectedly m...

The instability of ascorbic acid in weakly acid solutions has recently been shown to be due to the copper content of the solutions (1). By strict elimination of copper, solutions of ascorbic acid are stable below pH 7.6. The preparation of copper-free reagents, however, is not very practical and in analytical procedures would not eliminate copper contamination from the materials analyzed. The u...

BACKGROUND Ascorbic acid is a potent antioxidant and is involved in many metabolic activities including collagen biosynthesis. In the present investigation, ascorbic acid and lipid peroxides were monitored in the blood and liver samples during the progression of experimentally induced hepatic fibrosis. METHODS Liver injury was induced by intraperitoneal injections of dimethylnitrosamine (DMN)...

introduction : pistachio (pistaciavera l.) is a tasty nut and a good source of nutrients. ithas a high content of numerous beneficial nutritive and bioactive compounds such as proteins, carbohydrate, moisture, vitamins, minerals, fiber and other micronutrients compounds, but the most exceptional components are the amount of fats and especially unsaturated fatty acids. therefore, this nut is hig...

The effect of calcium chloride brine treatment on firmness and retention of phytochemicals in pastuerized yellow banana peppers was studied. Shear force values declined during processing and storage, but CaCl(2) treatment resulted in greater firmness retention. Processing reduced ascorbic acid content by 63%, and after 124 days, <10% of ascorbic acid remained. Quercetin and luteolin contents de...

OBJECTIVE Investigated the effect and mechanism of ascorbic acid on the development of porcine embryos produced by somatic cell nuclear transfer (SCNT). METHODS Porcine embryos were produced by SCNT and cultured in the presence or absence of ascorbic acid. Ten-eleven translocation 3 (TET3) in oocytes was knocked down by siRNA injection. After ascorbic acid treatment, reprogramming genes were ...

1. The effect ofdietary molybdenum on the growth rate and also on ascorbic acid metabolism in rats was studied. An excess of dietary molybdenum resulted in growth retardation and loss of weight. Tolerance to molybdenum was affected by the nature ofthe molybdenum salt administered. 2. Molybdenum ingestion altered certain aspects of ascorbic acid metabolism in rats. The conversion ofD-glucuronola...

Organic acids play pivotal role in flavor and consumer acceptance of fruit beverages. Concentration of some organic acids in citrus juices was determined through HPLC and their retention during refrigerated storage was also evaluated. The concentration of organic acids were in the following pattern Citric Acid> Malic Acid> Ascorbic Acid> Tartaric Acid. Citric acid, malic acid, ascorbic acid and...

The strawberry cultivars Arosa, Clery, Miss, Raurica, Queen Elisa, Diamante and Madeleine grown in Slovenia were harvested at the commercially maturity stage and the content of total ascorbic acid and dehydroascorbic acid (DHA) was determined. Ascorbic acid (AA) was measured by using high-performance liquid chromatography and photodiode array detector. The total AA content of cultivars ranged f...