Current therapies for sarcomas are often inadequate. This study sought to identify actionable gene targets by selective targeting of the molecular networks that support sarcoma cell proliferation. Silencing of asparagine synthetase (ASNS), an amidotransferase that converts aspartate into asparagine, produced the strongest inhibitory effect on sarcoma growth in a functional genomic screen of mou...

L-Asparagine is responsible for a major portion of the effect of peptone on the growth of L5178Y lymphoblasts in culture and is essential for the reproduction of these cells. Among the compounds tested, the requirement for the amide is replaced only by certain peptide derivatives of asparagine, and these are less active on a molar basis than is L-asparagine; L-aspartic acid, D-asparagine, and L...

Free asparagine in cereals is known to be the precursor of acrylamide, a neurotoxic and carcinogenic product formed during cooking processes. Thus, the development of crops with lower asparagine is of considerable interest to growers and the food industry. In this study, we describe the development and application of a rapid (1)H-NMR-based analysis of cereal flour, that is, suitable for quantif...

Since its discovery in many heat-treatment foods 2002, efforts have been made to reduce acrylamide levels foods. Methods by reducing Maillard reaction products considered. However, baking cookies produces acrylamide, a carcinogenic compound. This study aimed use new quantitative index and formula for L-asparaginase, glucose oxidase, their 1:1 blending enzymes, baker’s yeast, green tea powder (0...

Magic-angle single and double cross-polarization (13)C and (15)N NMR spectra have been obtained of lyophilized soybean cotyledons cultured on media containing, as the only nitrogen source, [4-(13)C, amide-(15)N]asparagine. Single cross-polarization NMR shows directly and unambiguously that both labels from asparagine are incorporated extensively and nonrandomly into protein by the developing co...