The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP s...

We report a case of Aspergillus terreus discitis which developed in a patient with acute lymphoblastic leukemia following induction chemotherapy. A. terreus was isolated from sputum, one month earlier, but the physician did not consider it significant at the time. Magnetic resonance imaging study showed the involvement of L3-4, L4-5 and L5-S1 intervertebral discs. Etiology was established by me...

The in vitro effect of water activity (0.995, 0.98, 0.95, 0.90 and 0.85) and temperature (25 and 15 degrees C) on competing abilities of the biocontrol agent Penicillium oxalicum against Fusarium oxysporum fsp. lycopersici, a tomato pathogen, and Fusarium oxysporum fsp. gladioli, a gladiolus pathogen, was evaluated. The aim of this study was to assess the suitability of P. oxalicum to be applie...

The solid waste of sago industry using cassava was fermented by Aspergillus niger, Aspergillus terreus and Rhizopus stolonifer in solid state fermentation. Cassava waste contained 52 per cent starch and 2.9 per cent protein by dry weight. The amylase activity was maintained at a high level and the highest amylase activity was observed on the 8(th) day in R. stolonifer mediated fermentation. R. ...

Sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. Cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-Czapek's agar at 28℃. The most common genera were Alternaria, Aspergillus, Emericella, Fusarium, Mycosphaerella, Nectria...

The inactivation of spores of Penicillium oxalicum by supercritical carbon dioxide (SC-CO2) was optimized by response surface methodology. The optimal inactivation conditions of 16.8 MPa, 49°C, and 20 min were determined using ridge analysis, at which the predicted and experimental log10 reductions were obtained as 5.74 and 6.12, respectively. The synergistic effect of a cosolvent (ethanol), wh...

PURPOSE Fungi have been known to be important aeroallergens for hundreds of years. Most studies have focused on total fungal concentration; however, the concentration of specific allergenic fungi may be more important on an individual basis. METHODS Ten fungal allergic patients and 2 non-fungal allergic patients were enrolled. The patients with a decrease in physician or patient global assess...