Assisted hatching (AH), which is known to improve the hatching potential of mammalian embryos, has been used to increase the pregnancy rate in in vitro fertilization cycles. However, the effect of AH on a trypsin-like protease, which is known to be associated with the hatching process, has not been studied. In this study, we evaluate whether the intactness of zona pellucida affects the secretio...

OBJECTIVE To analyze data from a large multicenter study to determine whether pregnancy and delivery rates decrease with repeated IVF-ET cycles. DESIGN Multicenter retrospective study. SETTING Participating centers from the Society of Assisted Reproductive Technology. PATIENT(S) Fifty-four centers contributed 4,043 cycles of oocyte retrieval for uterine transfer. INTERVENTION(S) Oocyte ...

At present, the problem of increasing effectiveness programs assisted reproductive technologies and successful infertility treatment is still relevant. Assisted hatching used in devitrificated embryo transfer facilitates exit from pellucide zone. Yet clinical efficacy relevant debatable. There are no clear indications for use this technology, groups patients have been identified. The aim study....

When a blastocyst expands with the growth of the blastocoel, it stretches and thins the zona pellucida (ZP), ultimately rupturing the ZP allowing the embryo to hatch free ready for implantation. In the event this does not occur, then an otherwise potentially viable embryo may not implant. Assisted hatching (AH) has been developed to overcome this, but has been applied mostly to day-2/3 embryos ...