Targeted resequencing of genomic DNA from organisms such as humans is an important tool enabling experimental access to variation within the species and between similar species. Taking full advantage of the reference genome sequences in designing robust, specific PCR assays and using stringent conditions, resequencing can be done efficiently without purification of the PCR product. By using a 1...

The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BE...

Tkáãiková a. , M. R. , Bhide I . Mikula: Asymmetric PCR-SSCP: a Useful Tool for Detection of OLA-DRB1(MHC class II) Gene Polymorphism in Slovak Improved Valachian Sheep. Acta Vet. Brno 2005, 74: 275-278. Detection of OLA-DBR1 (exon 2) gene polymorphism is presented in the paper. Rapid and inexpensive polymorphism detection method, namely, single stranded conformation polymorphism (SSCP) was ass...

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

In this paper, we propose a simple bilayered chiral matamaterial (CMM) consisting of two layers of split oval ring resonators by twisted angle of 90°. Numerical simulated results demonstrate that the proposed structure can realized a mutual polarization conversion and dual-band asymmetric transmission of linearly polarized waves in two opposite directions. The structure can convert the linearly...

...................................................................................................................................... I Chapter 1: General introduction and outline..............................................................................1 1.1. Blood coagulation system ............................................................................................... 2 1.2. Regu...

o bjectives: headgears are among the effective orthodontic appliances to achieve treatment goals. unilateral molar distal movement is sometimes needed during an orthodontic treat- ment, which can be achieved by an asymmetric headgear. different unilateral headgears have been introduced. the main goal of this study was to analyze the force system of uni- lateral expanded outer bow asymmetric hea...