نتایج جستجو برای: azf microdeletion

تعداد نتایج: 1732  

2010
Hakan Gurkan Faruk Kucukdurmaz Tolga Akman Filiz Aydın Ates Kadioglu

Genomic DNA of a patient diagnosed with nonobstructive azoospermia and with the history of allogenic bone marrow transplantation from his sister due to chronic myeloid leukemia was isolated from peripheral blood in order to screen Y chromosome microdeletions. 13 short tagged sites belonging to AZF a, b, and c loci were detected with multiplex polymerase chain reaction technique. Bands were dete...

Journal: :International journal of andrology 2004
M Simoni E Bakker C Krausz

Microdeletions of the Y chromosome are the second most frequent genetic cause of spermatogenetic failure in infertile men after the Klinefelter syndrome. The molecular diagnosis of Y-chromosomal microdeletions is routinely performed in the workup of male infertility in men with azoospermia or severe oligozoospermia. Since 1999, the European Academy of Andrology (EAA) and the European Molecular ...

Journal: :Human molecular genetics 1996
P H Vogt A Edelmann S Kirsch O Henegariu P Hirschmann F Kiesewetter F M Köhn W B Schill S Farah C Ramos M Hartmann W Hartschuh D Meschede H M Behre A Castel E Nieschlag W Weidner H J Gröne A Jung W Engel G Haidl

In a large collaborative screening project, 370 men with idiopathic azoospermia or severe oligozoospermia were analysed for deletions of 76 DNA loci in Yq11. In 12 individuals, we observed de novo microdeletions involving several DNA loci, while an additional patient had an inherited deletion. They were mapped to three different subregions in Yq11. One subregion coincides to the AZF region defi...

Journal: :Indian journal of experimental biology 2005
Kiran Singh Rajiva Raman

The spermatogenesis locus azoospermia factor (AZF) in Yq11 has been delineated into three microdeletion intervals designated as AZFa, AZFb andAZFc. AZFc is the most frequently deleted region. We have studied 270 male infertile patients for various genetic disorders associated with infertile phenotype. In this study, we have presented results of our studies on Y-chromosome deletions, chromosomal...

Journal: :Asian journal of andrology 2006
Anurag Mitra Rima Dada Rajeev Kumar Narmada Prasad Gupta Kiran Kucheria Satish Kumar Gupta

AIM To study the occurrence of Y chromosome microdeletions in azoospermic patients with Klinefelter's syndrome (KFS). METHODS Blood and semen samples were collected from azoospermic patients with KFS (n = 14) and a control group of men of proven fertility (n = 13). Semen analysis was done according to World Health Organization (WHO) guidelines. Blood samples were processed for karyotyping, fl...

Journal: :Fertility and sterility 2004
Janet M Choi Pak Chung Lucinda Veeck Anna Mielnik Gianpiero D Palermo Peter N Schlegel

OBJECTIVE To determine whether the presence of a Y microdeletion confers any adverse effects on in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) outcome. DESIGN Retrospective case-control study. SETTING Academic infertility center. PATIENT(S) A total of 17 patients with Y microdeletions who attempted IVF/ICSI cycles at our center between March 1996 and March 2002 were...

2017
Prafulla S. Ambulkar Sunil S. Pande

Genetic factors cause about 15% of male infertility and microdeletions of Y chromosome is one of the genetic causes in idiopathic infertile men. Azoospermia factors (AZFa, AZFb, and AZFc) on Yq long arm are most important for spermatogenesis. For analysis of microdeletions in the AZF regions by sequence-tagged-site (STS) PCR is important screening method for infertility. An attempt has been mad...

Journal: :Genetics and molecular research : GMR 2013
H-G Zhang Z-B Zhang R-X Wang Y Yu X-W Yu E Fadlalla R-Z Liu

The prevalence of microdeletions of azoospermia factor (AZF) among azoospermic Klinefelter's syndrome (KFS) patients shows conflicting data. We aimed to detect this frequency in a Northeast Chinese population, and to investigate the possible association between AZF microdeletions and KFS by comparison with previous conflicting reports. Eighty men affected with KFS and a random healthy control g...

Journal: :International Journal of Andrology 2008

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