Complete sequential 1H and 15N resonance assignments for the reduced Cu(I) form of the blue copper protein azurin (M(r) 14,000, 128 residues) from Pseudomonas aeruginosa have been obtained at pH 5.5 and 40 degrees C by using homo- and heteronuclear two-dimensional (2D) and three-dimensional (3D) nuclear magnetic resonance spectroscopic experiments. Combined analysis of a 3D homonuclear 1H Hartm...

24 Stenotrophomonas maltophilia is an important global opportunistic pathogen for which 25 limited therapeutics are available because of the emergence of multidrug-resistant strains. 26 A novel bacteriocin, maltocin P28, which is produced by S. maltophilia strain P28, is the 27 first-identified phage tail-like bacteriocin from S. maltophilia. Maltocin P28 resembles a 28 contractile but non-flex...

Stenotrophomonas maltophilia is an important global opportunistic pathogen for which limited therapeutics are available because of the emergence of multidrug-resistant strains. A novel bacteriocin, maltocin P28, which is produced by S. maltophilia strain P28, may be the first identified phage tail-like bacteriocin from S. maltophilia. Maltocin P28 resembles a contractile but nonflexible phage t...

The 28,000-dalton (p28) major structural polypeptide of the mouse mammary tumor virus (MMTV) was isolated and used to develop a highly sensitive and specific radioimmunoassay. Under conditions of limiting antibody in competitive binding assays, as little as 50 pg of purified p28, as well as disrupted MMTV virions and mammary tumor extracts, competed specifically with 125I-labeled MMTV p28. The ...

We report the identification and cloning of a 28-kDa polypeptide (p28) in Tetrahymena macronuclei that shares several features with the well studied heterochromatin-associated protein HP1 from Drosophila. Notably, like HP1, p28 contains both a chromodomain and a chromoshadow domain. p28 also shares features with linker histone H1, and like H1, p28 is multiply phosphorylated, at least in part, b...

Oxidation (off state) and reduction (on state) of a single azurin molecule is monitored, one electron at a time, which depend on the chemical redox potential. By analysing the fluorescence time traces from individual azurin molecules, reaction kinetics and redox thermodynamics were determined.

The long-range intramolecular electron transfer (ET) rates between the disulfide radical anion and the copper site in azurin from Alcaligenes denitrificans and three mutants of azurin from A. denitrificans and Pseudomonas aeruginosa have been measured. In the three mutants a copper ligand of azurin was substituted by a glycine (His46Gly and His117Gly azurin) or a histidine (Met121His azurin). M...

Mycobacterium tuberculosis remains a major global challenge to human health care, and the mechanisms of how M. tuberculosis evades host immune surveillance to favor its survival are still largely unknown. In this study, we found that bacillus Calmette-Guérin (BCG) and viable M. tuberculosis as well as M. tuberculosis lysates could activate IL-27 expression in human and mouse macrophages by indu...

Azurin proteins are the workhorse of protein electronics. This is a branch biomolecular electronics, recent research field which investigates electronics based on biomolecules such as proteins, peptides, amino acids, bacterial nanowires or DNA. In general, possibility including biosystems in solid-state junctions has opened way to development novel electrical devices, and have attracted enormou...

To characterize fibroblast growth factor (FGF) gene expression in the late fetal (days E18 to E22) and early postnatal lung (days P0 to P28), when the alveolar region undergoes extensive growth and reorganization, we analyzed the expression of four FGF receptors and six ligands. FGF receptor 1 (FGFR1) RNA levels were first low (E18) before rising late in the postnatal period (P28). FGFR2 RNA le...