Droplet digital polymerase chain reaction (ddPCR) is a novel molecular biology technique providing absolute quantification of target nucleic acids without the need for an external calibrator. Despite its emerging applications in medical diagnosis, there are few reports of its use for the detection of plant pathogens. This work was designed to assess the diagnosis potential of the ddPCR for abso...

Digital image processing is employed in numerous areas of biology to identify and analyse problems. This approach aims to use image processing techniques for citrus canker disease detection through leaf inspection. Citrus canker is a severe bacterium-based citrus plant disease. The symptoms of citrus canker disease typically occur in the leaves, branches, fruits and thorns. The leaf images show...

Bacterial canker caused by the Gram-positive actinobacterium Clavibacter michiganensis is one of most serious bacterial diseases tomatoes, responsible for 10–100% yield losses worldwide. The pathogen can systemically colonize tomato vascular bundles, leading to wilting, cankers, bird’s eye lesions, and plant death. Bactericidal agents are insufficient managing this disease, because rapidly migr...

Of all the agricultural pests and diseases that threaten citrus crops, citrus canker is one of the most devastating. The disease, caused by the bacterium Xanthomonas axonopodis pv. citri, occurs in large areas of the world's citrus growing countries including India. At least 3 distinct forms or types of citrus canker are recognized. Among these, Asiatic form (Canker A) is the most destructive a...

Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating biotic stresses affecting the citrus industry. Meiwa kumquat (Fortunella crassifolia) is canker-resistant, while Newhall navel orange (Citrus sinensis Osbeck) is canker-sensitive. To understand the molecular mechanisms underlying the differences in responses to Xcc, transcriptomic profiles of the...

Epidemiology studies of Pseudomonas syringae pathovars associated with bacterial canker on the sweet cherry in Serbia | Renata Iličić, Jelica Balaž, Vladislav Ognjanov, Tatjana Popović Agricultural Journals

A bacterial pathogen, Pseudomonas syringae pv. actinidiae (Psa), is a causal agent of kiwifruit bacterial canker worldwide. Psa biovar 3 (Psa3) was first detected in 2011 at an orchard in Dodeok-myeon, Goheunggun, Jeonnam Province in Korea. In this study, we present the results of an epidemiological study regarding Psa3 occurrence on kiwifruit orchards in Korea for the period of 2013 to 2015. S...

samples of leaf, twig and fruit from ‘mexican’ lime (citrus aurantifolia) and grapefruit (citrus paradisi) with symptoms of bacterial canker were collected from different provinces throughout iran during spring and summers of 2010 and 2011. yellow, gram-negative colonies were isolated from infected tissue samples. results of pathogenicity assays indicated that some isolates incited tissue hyper...

Citrus (family Rutaceae) stands as a prominent fruit crop on global scale, bearing substantial significance. Renowned for its abundance of fiber, amino acids, antioxidants, vitamin C, and carbohydrates. However, the successful production citrus is persistently challenged by menacing presence canker caused Xanthomonas citri subsp. (Xcc), posing 5-30% yield losses in Pakistan. Current study was a...