نتایج جستجو برای: baculovirus expression system

تعداد نتایج: 2988560  

Journal: :The Biochemical journal 1996
S A Ali H C Joao R Csonga F Hammerschmid A Steinkasserer

Recently, there has been much interest in expressing recombinant human serum transferrin (HST) and mutants thereof for structural and functional studies. We have developed a baculovirus expression system for the rapid and efficient production of large quantities of HST (> 20 mg/l). Like native HST, the recombinant protein can bind two ferric ions in the presence of bicarbonate, and is actively ...

2005
Marc LOMBES Nadine BINART Francine DELAHAYE Etienne Emile BAULIEU

The effect of aldosterone and antimineralocorticoids on the intracellular localization of human mineralocorticosteroid receptor (hMR) was studied using a new monoclonal anti-peptide antibody FD4. This antibody was directed against the peptide hMR-(412-422). As demonstrated by ultracentrifugation analysis, immunoprecipitation assays and Western blot, FD4 recognized both the native and denatured ...

Journal: :BioTechniques 2004
Björn Philipps Michael Forstner Lorenz M Mayr

Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (BEVS) is hampered by slow and tedious procedures for the selection and separation of baculovirus-infected insect cells and for titer determination. Here we developed a new tech...

Journal: :Turkish Journal of Biology 2021

RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that required for gene expression. Gdown1 containing Pol [Pol (G)] special form of catalytically inactive and heavily depends on the 30-subunit Mediator its activator basal dependent function in vitro. Here we report first time, identification generation 15-subunit via novel multibac baculovirus expression system ful...

Journal: :BioTechniques 2006
Qi Ma Li Zhou Lixin Ma Keke Huo

The baculovirus expression vector system (BEVS) has become one of the most widely used systems for routine protein expression. We have developed an improved strategy to clone foreign genes directionally and directly into the baculovirus genome vector via a one-step procedure to generate recombinant viruses in a week. In this work, we constructed a host strain Escherichia coli DH10BacHB1.1, whic...

2010
Alisson G Lynch Fiona Tanzer Malcolm J Fraser Enid G Shephard Anna-Lise Williamson Edward P Rybicki

BACKGROUND Insect baculovirus-produced Human immunodeficiency virus type 1 (HIV-1) Gag virus-like-particles (VLPs) stimulate good humoral and cell-mediated immune responses in animals and are thought to be suitable as a vaccine candidate. Drawbacks to this production system include contamination of VLP preparations with baculovirus and the necessity for routine maintenance of infectious baculov...

2017
Zehui Liu Yangkun Liu Yuanyuan Zhang Yajuan Yang Jingjing Ren Xiaoying Zhang Enqi Du

Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application of baculovirus. A variety of approaches have been exploited to bypass the complement system in the serum. In this study, we constructed and sc...

Journal: :Molecular biotechnology 2007
Kevin P O'Connell Elena Kovaleva James H Campbell Patricia E Anderson Susan G Brown David C Davis James J Valdes Richard W Welch William E Bentley Nikolai A van Beek

Infection of insect cells with baculovirus expression constructs is commonly used to produce recombinant proteins that require post-translational modifications for their activity, such as mammalian proteins. However, technical restraints limit the capacity of insect cell-based culture systems to be scaled up to produce the large amounts of recombinant protein required for human pharmaceuticals....

Journal: :iranian journal of basic medical sciences 0
mahdi rouhbakhsh blood transfusion research centre, high institute for research and education in transfusion medicine, tehran, iran raheleh halabian blood transfusion research centre, high institute for research and education in transfusion medicine, tehran, iran nasser masroori blood transfusion research centre, high institute for research and education in transfusion medicine, tehran, iran mahshid mohammadi pour blood transfusion research centre, high institute for research and education in transfusion medicine, tehran, iran parisa bahmani blood transfusion research centre, high institute for research and education in transfusion medicine, tehran, iran amaneh mohammadi roushandeh department of anatomy, faculty of medicine, medical university of hamadan, hamadan, iran

objective(s) lipocalin 2 (lcn2) is a 25-kda glycoprotein that has initially been extracted from neutrophil granules. expression of lcn2 is induced under various pathophysiological conditions. it is also known as an early marker of kidney and heart injury. high-level expression of recombinant lcn2 neutrophil gelatinase-associated(ngal) in insect cells was the aim of this study. materials and met...

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