Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy demanding cells. However, the understanding of how respiration is globally regula...

These studies explore the consequences of activating the prolyl hydroxylase (PHD) O(2)-sensing pathway in spontaneously twitching neonatal cardiomyocytes. Full activation of the PHD pathway was achieved using the broad-spectrum PHD inhibitor (PHI) dimethyloxaloylglycine (DMOG). PHI treatment of cardiomyocytes caused an 85% decrease in O(2) consumption and a 300% increase in lactic acid producti...

When washed at moderate temperatures, slices of many underground storage organs show a steady rise in their rate of respiration until it reaches a value two to five times that of freshly cut tissue. Laties (3) has recently reviewed our knowledge of these changes in respiration. In accordance with his suggestion, the respiration which develops gradually on washing will be referred to as induced ...

OBJECTIVE To identify relationships between insulin resistance (IR) and mitochondrial respiration in perinatally HIV-infected youth. DESIGN Case-control study. METHODS Mitochondrial respiration was assessed in perinatally HIV-infected youth in Tanner stages 2-5, 25 youth with IR (IR+) and 50 without IR (IR-) who were enrolled in the Pediatric HIV/AIDS Cohort Study. IR was defined as a homeo...

Eddy covariance nighttime fluxes are uncertain due to potential measurement biases. Many studies report eddy covariance nighttime flux lower than flux from extrapolated chamber measurements, despite corrections for low turbulence. We compared eddy covariance and chamber estimates of ecosystem respiration at the GLEES Ameriflux site over seven growing seasons under high turbulence [summer night ...

Aim Quantify direct and indirect relationships between soil microbial community properties (potential basal respiration, biomass) abiotic factors (soil, climate) in three major land-cover types. Location Europe. Time period 2018. Major taxa studied Microbial (fungi bacteria). Methods We collected 881 samples from across Europe the framework of Land Use/Land Cover Area Frame Survey (LUCAS). meas...

This Application Note describes a method for profiling mitochondrial function in cells responding to stress. The mitochondrial profile generated in this way provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity. The results described here suggest that the development of cardiom...