The objective of the current study was to evaluate the reproducibility of a reverse transcriptase PCR (RT-PCR)-based technique to differentiate viable and dead Salmonella cells in raw and sterilized milk. The microorganism was initially inoculated into the milk samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80°C for 10 min. The treated and non-treated samples we...

MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3'-end length variants ("isomiRs"), little attention has been paid to their differential detection by RT-qPCR. However, recent evidence indicates that 3'-end miRNA i...

Several technologies, such as electromagnetic midsagittal articulography (EMA) or real-time magnetic resonance (RTMRI), enable studying the static and dynamic aspects of speech production. The resulting knowledge can, in turn, inform the improvement of speech production models, e.g., for articulatory speech synthesis, by enabling the identification of which articulators and gestures are involve...

This paper considers inventory models with advance demand information and flexible delivery. Customers place their orders in advance, and delivery is flexible in the sense that early shipment is allowed. Specifically, an order placed at time t by a customer with demand leadtime T should be fulfilled by period t+T ; failure to fulfill it within the time window [t, t+T ] is penalized. We consider...

statement of problem: condylar hyperplasia of the mandible is a self limiting abnormality which can cause facial asymmetry, temporomandibular joint (tmj) dysfunction and esthetic problems. treatment planning is based on the results of isotope scanning, clinical findings and patient age. single photon emission tomography (spect) is considered to be a sensitive method in the calculation of condyl...

abstract background and objective: highly active antiretroviral therapy (haart) can effectively prevent the progression of hiv-1 replication and increase life expectancy. there are numerous causes of treatment failure and the leading one is drug resistance. thus, we aimed to determine the hiv rt gene drug resistance mutations in patients treated with antiretroviral medications. material and met...

This paper presents an improvement of the Bandwidth Inheritance Protocol (BWI), the natural extension of the well-known Priority Inheritance Protocol (PIP) to resource reservation schedulers. The modified protocol allows for a better management of nested critical section, removes unneeded overheads in the management of task block and unblock events, and introduces a run-time deadlock detection ...