Unbleached pulp from the pulp and paper industry was used as a cellulosic material source to produce bioethanol in a separate hydrolysis and fermentation process. In the batch enzymatic saccharification, three cellulolytic enzymes and different initial carbohydrate (CH) contents were tested. Cellic CTec2 was shown to be the most efficient enzyme with the lowest dosage used: 35 FPU gCH . Sugar p...

Improved production costs will accelerate commercialization of polyhydroxyalkanoate (PHA) polymer and PHA-based products. Plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. Different PHA production strategies were compared using a recombinant strain of Ralstonia eutropha that produces ...

Brazilian ethanol fermentation process commonly uses baker's yeast as inoculum. In recent years, wild type yeast strains have been widely adopted. The two more successful examples are PE-2 and CAT-1, currently employed in Brazilian distilleries. In the present study, we analyzed how these strains compete for nutrients in the same environment and compared the potential characteristics which affe...

Background Lignin valorization has recently been considered to be an essential process for sustainable and cost-effective biorefineries. Lignin represents a potential new feedstock for value-added products. Oleaginous bacteria such as Rhodococcus opacus can produce intracellular lipids from biodegradation of aromatic substrates. These lipids can be used for biofuel production, which can potenti...

Improved production costs will accelerate commercialization of polyhydroxyalkanoate (PHA) polymer and PHA-based products. Plant oils are considered favorable feedstocks, due to their high carbon content and relatively low price compared to sugars and other refined carbon feedstocks. Different PHA production strategies were compared using a recombinant strain of Ralstonia eutropha that produces ...

Fermentation is the most time consuming step in the production of beer and therefore the effective use of fermentation vessels is a crucial element in brewing economy. One means of increasing the productivity of a batch process is to convert it to a continuous one. Experiments in continuous fermentation emerged during the 1950s and 1960s, but by the end of 1970s most of them had been closed dow...

abstract human insulin-like growth factor i (higf-i) is a kind of growth factor with clinical significance in medicine. the major objective of this study is over- production of recombinant human insulin-like growth factor i( rhigf-i) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. up to now e. coli expression system has been widely us...

one of the major obstacles of bt production as a biopesticide is its expensive bioprocess and fermentation. therefore, the objective of the present study was to optimize growth condition and develop a low-cost bioprocess for mass production of a native coleopteran-effective bacillus thuringiensis (bt) strain (kh4) based on agricultural wastes, at incubator and batch fermenter level. preliminary...

A repeated batch fermentation system was used to produce ethanol using Saccharomyces cerevisiae strain (NCIM 3640) immobilized on sugarcane (Saccharum officinarum L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsu...

This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bi...