نتایج جستجو برای: bead array

تعداد نتایج: 140843  

2016
Burcu Ayoglu Elin Birgersson Anja Mezger Mats Nilsson Mathias Uhlén Peter Nilsson Jochen M. Schwenk

Antibody microarrays enable parallelized and miniaturized analysis of clinical samples, and have proven to provide novel insights for the analysis of different proteomes. However, there are concerns that the performance of such direct labeling and single antibody assays are prone to off-target binding due to the sample context. To improve selectivity and sensitivity while maintaining the possib...

2014
Alexander Nesterov-Mueller Frieder Maerkle Lothar Hahn Tobias Foertsch Sebastian Schillo Valentina Bykovskaya Martyna Sedlmayr Laura K. Weber Barbara Ridder Miriam Soehindrijo Bastian Muenster Jakob Striffler F. Ralf Bischoff Frank Breitling Felix F. Loeffler

In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughp...

Journal: :Analytical chemistry 2001
K J Albert D R Walt D S Gill T C Pearce

A fiber optic bead-based sensor array platform has been employed to discriminate between six different odors and air carrier gas. Six different bead sensor types, with over 250 replicates of each, were monitored before, during, and after odor exposure to produce time-dependent fluorescence response patterns that were unique for each sensor-analyte combination. A total of 2,683 sensors were anal...

2013
Michael S. Akhras Erik Pettersson Lisa Diamond Magnus Unemo Jennifer Okamoto Ronald W. Davis Nader Pourmand

Here we describe the novel Sequencing Bead Array (SBA), a complete assay for molecular diagnostics and typing applications. SBA is a digital suspension array using Next-Generation Sequencing (NGS), to replace conventional optical readout platforms. The technology allows for reducing the number of instruments required in a laboratory setting, where the same NGS instrument could be employed from ...

Journal: :Current Biology 2004
Julie Plastino Stéphane Olivier Cécile Sykes

For cells, the growth of a dense array of branched actin filaments organized by the actin-related proteins 2 and 3 (Arp2/3) complex at the plasma membrane offers an explanation as to how movement is produced, and this arrangement is considered to be optimal for motility. Here, we challenged this assumption by using an in vitro system of polystyrene beads in cell extracts that contained a comple...

Journal: :Sensors 2018
Guillermo Alvarez Bestard Renato Coral Sampaio José A R Vargas Sadek C Absi Alfaro

The arc welding process is widely used in industry but its automatic control is limited by the difficulty in measuring the weld bead geometry and closing the control loop on the arc, which has adverse environmental conditions. To address this problem, this work proposes a system to capture the welding variables and send stimuli to the Gas Metal Arc Welding (GMAW) conventional process with a con...

Journal: :international journal of industrial mathematics 2014
a. razani

the notion of a bead metric space is defined as a nice generalization of the uniformly convex normed space such as $cat(0)$ space, where the curvature is bounded from above by zero. in fact, the bead spaces themselves can be considered in particular as natural extensions of convex sets in uniformly convex spaces and normed bead spaces are identical with uniformly convex spaces. in this paper, w...

Journal: :Molecular ecology resources 2012
Lani U Gleason Ronald S Burton

The location and abundance of fish eggs provide information concerning the timing and location of spawning activities and can provide fishery-independent estimates of spawning biomass. However, the full value of egg and larval surveys is severely restricted because many species' eggs and larvae are morphologically similar, making species-level identification difficult. Recent efforts have shown...

Journal: :Chemical communications 2009
Huan Huang Zongtai Qi Lili Deng Guohua Zhou Tomoharu Kajiyama Hideki Kambara

In order to detect small amounts of mutants for early cancer diagnosis, we have developed the novel method of using amplicon-coated microbeads and single-molecule-PCR in water-in-oil emulsions, which we coupled with a new detection platform, the hydrogel bead-array, a 3-D polyacrylamide gel network used as a carrier to immobilize the beads.

Journal: :Journal of clinical microbiology 2011
Y Wu E L Tan A Yeo K P Chan H Nishimura M J Cardosa C L Poh S H Quak Vincent T Chow

A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.

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