Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated ...

Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3-5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige ...

BACKGROUND Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. OBJECTIVES In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated. MATERIALS AND ...

Yeast cells expressing cDNA libraries have provided two new approaches to facilitate further identification of cytokinin-binding proteins and receptors. These are the yeast three hybrid (Y3H) system and fluorescence activated cell sorting (FACS). The Y3H system requires a synthetic hybrid ligand comprising an "anchor" moiety (e.g., dexamethasone) linked to a cytokinin via a spacer. In the yeast...

Embryo rescue refers to an in vitro technology have been used for propagating plants under from poorly developed embryos by avoiding embryo abortion. The aim of the present study was regeneration a medicinally wild tree species Buchanania lanzan through method. Isolated immature dissected out de-coded seeds collected 15 years old trees were cultured Woody Plant Medium (WPM) test germination. 10...

Salicylic acid (SA) is one of numerous phenolic compounds found in plants with numerous important physiological events. Exogenously application of Salicylic acid (SA) is useful for the growth and development of plants. This investigation aimed to study the improving effect of salicylic acid on the tissue culture of Ziziphus spina-christi. To study its improving effect were used shoot-tip explan...

The objective of this study was to investigate the effect cytokinins benzylaminopurine (BAP) and Kinetin on in vitro shootregeneration shoot tips Polyscias balfouriana marginata. Shoot were cultured Murashige Skooge (1962) medium (MS) supplemented with various concentrations BAP at 0.0, 0.75, 1.5, 3, 6 mg/L for both. Results showed that marginata responded positively growth regulators applicati...

The present study focuses on the development of a micropropagation protocol for true to type plants Anubias heterophylla, valuable ornamental aquatic plant. Natural propagation species is limited due production small number with long cultivation period, disease and requirement large space propagation. Surface-disinfected shoot tip explants were cultured MS medium containing 0.1 % (w/v) activate...

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

In this study, it was aimed to determine the total antioxidant capacity and phenolic flavonoid amounts of Lavandula officinalis (lavender), which is an aromatic plant, callus extracts. Combinations Naphthalene Acetic Acid (NAA), Benzylaminopurine (BAP), Kinetin (KIN) 2,4-Dichlorophenoxy (2,4-D) plant growth regulators at different concentrations were used in medium. The specified analyzes carri...