BACKGROUND In mammals, the CLOCK/BMAL1 heterodimer is a key transcription factor complex that drives the cyclic expression of clock-controlled genes involved in various physiological functions and behavioral consequences. Recently, a growing number of studies have reported a molecular link between the circadian clock and metabolism. In the present study, we explored the regulatory effects of SI...

The bimolecular fluorescence complementation (BiFC) assay has been widely accepted for studying in vivo detection of protein-protein interactions in several organisms. To facilitate the application of the BiFC assay to yeast research, we have created a series of plasmids that allow single-step, PCR-based C- or N-terminal tagging of yeast proteins with yellow fluorescent protein fragments for Bi...

The renewable energy sources and plug-in electric vehicles (PEVs) are becoming very popular because of the combination of high fuel costs and concerns about emission issues. This paper presents modelling and control of a Building Integrated Fuel Cell and Plug-in Electric Vehicles (BIFC-PEV) in smart distribution systems. In BIFC-PEV system, conventional building elements could be replaced by sp...

We present a high-throughput approach to study weak protein-protein interactions by coupling bimolecular fluorescent complementation (BiFC) to flow cytometry (FC). Bimolecular Fluorescence Complementation (BIFC) is a method to detect proteinprotein interactions based on the formation of a fluorescent complex by fragments of the yellow fluorescent protein (NYFP and CYFP) brought together by the ...

The twin-arginine translocation (Tat) pathway is well known for its ability to export fully folded substrate proteins out of the cytoplasm of gram-negative and gram-positive bacteria. Studies of this mechanism in Escherichia coli have identified numerous transient protein-protein interactions that guide export-competent proteins through the Tat pathway. To visualize these interactions, we have ...

Bimolecular fluorescence complementation (BiFC) represents one of the most advanced and powerful tools for studying and visualizing protein-protein interactions in living cells. In this method, putative interacting protein partners are fused to complementary non-fluorescent fragments of an autofluorescent protein, such as the yellow spectral variant of the green fluorescent protein. Interaction...

embryogenesis. We extended this analysis to other Hox-Exd, or Hox-Hox interactions, revealing specific spatial and temporal dynamics during embryogenesis. Altogether, these results show that the BiFC is a highly sensitive method for analysing protein– protein interactions in the living drosophila embryo. Based on the BiFC approach, we established two genetic strategies to find novel cofactors o...

embryogenesis. We extended this analysis to other Hox-Exd, or Hox-Hox interactions, revealing specific spatial and temporal dynamics during embryogenesis. Altogether, these results show that the BiFC is a highly sensitive method for analysing protein– protein interactions in the living drosophila embryo. Based on the BiFC approach, we established two genetic strategies to find novel cofactors o...

There is considerable evidence to support the existence of dimers of class A G-protein-coupled receptors, including the histamine H1 receptor (H1R) (Bakker et al, 2004), although the functional significance of these dimers remains unclear. We have used bimolecular fluorescence complementation (BiFC) in combination with fluorescence correlation spectroscopy (FCS) to selectively monitor histamine...

embryogenesis. We extended this analysis to other Hox-Exd, or Hox-Hox interactions, revealing specific spatial and temporal dynamics during embryogenesis. Altogether, these results show that the BiFC is a highly sensitive method for analysing protein– protein interactions in the living drosophila embryo. Based on the BiFC approach, we established two genetic strategies to find novel cofactors o...