Bile acids make up a group of sterol-derived compounds that act as detergents in the intestine to facilitate the digestion and absorption of fats and fat-soluble molecules. In mammalian species, the cholesterol side chain is trimmed to yield C24-sterol derivatives. In other vertebrate species, the hydroxylation of the side chain does not lead to its removal and the products of the biosynthetic ...

In this study, we compared in vitro calcium binding by the taurine and glycine conjugates of the major bile acids in human bile: cholic (CA), chenodeoxycholic (CDCA) and deoxycholic (DCA) acids, together with the cholelitholytic bile acids ursodeoxycholic (UDCA) and ursocholic (UCA) acids. At physiological total calcium (CaTOT) (1-15 mM) and bile acid (BA) (10-50 mM) concentrations, all the bil...

For a long time, it has been assumed that the liver plays an important role in the formation and excretion of bile acids and that the metabolism of bile acids, therefore, can be disturbed to some extent in liver diseases. Pathophysiological aspects of the disturbed metabolism of bile acids remained obscure at the time when procedures of qualitative and quantitative analTses, identification and ...

Fecal microbiota transplantation (FMT) has emerged as a highly effective therapy for refractory, recurrent Clostridium difficile infection (CDI), which develops following antibiotic treatments. Intestinal microbiota play a critical role in the metabolism of bile acids in the colon, which in turn have major effects on the lifecycle of C. difficile bacteria. We hypothesized that fecal bile acid c...

A method is described for the fluorometric determination of fecal bile acids. The method combines solvent extraction and thin-layer chromatography (TLC) isolation tech nics with a fluorometric method for measuring total bile acids. Recoveries of individual bile acids added to feces averaged 107% with a standard deviation of ± 5.6%. Bile acid excretion in normal human subjects on a self-selected...

Using gas chromatography, the qualitative composition and quantitative content of bile acids in lipophilic complexes animal was studied. As a result studies, 6 steroids were identified bile, 5 which are acids. It defined that cholic deoxycholic acid predominate all studied objects, while their amount depends on used raw materials.

Absorption of conjugated bile acids from the small intestine is very efficient. The mechanisms of jejunal absorption are not very well understood. The aim of this study was to clarify the mechanism of absorption of conjugated bile acid at the apical membrane of jejunal epithelial cells. Brush-border membrane vesicles from intestinal epithelial cells of the rat were prepared. Absorption of two t...

Bile from Alligator mississippiensis was found to contain a mixture of more than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and bile alcohols were purified by reversed-phase high performance liquid chromatography and thin-layer chromatography. Concentrations were measured by gas-liquid chromatography on 1% HiEFF-8BP and identifications were made by mass spectrometry. The ...

Bile acids are steroidal amphipathic molecules derived from the catabolism of cholesterol. They modulate bile flow and lipid secretion, are essential for the absorption of dietary fats and vitamins, and have been implicated in the regulation of all the key enzymes involved in cholesterol homeostasis. Bile acids recirculate through the liver, bile ducts, small intestine and portal vein to form a...

INTRODUCTION: Bile acids, phospholipids, and cholesterol are the major lipid components in human bile [1]. The composition of bile is altered in various chronic cholestatic diseases and determining such alterations will be of great clinical importance in understanding the pathophysiology of these diseases [2]. Although there are a few chromatographic [3] and NMR-based methods [4,5] available fo...