Our laboratory has characterized androgen metabolism in an androgen-responsive prostate cancer cell line (LNCaP) and showed that these cells accumulated intracellular testosterone primarily as glucuronidated metabolites. Using a cell-free assay with testosterone as substrate, we showed that LNCaP had UDP-glucuronosyltransferase (UDPGT) activity. Because dietary factors, such as flavonoids in so...

Compounds that target the peroxisome proliferator-activated receptors PPARa and PPARg are used to correct dyslipidemia and to restore glycemic balance, respectively. Because the majority of diabetic patients suffer from atherogenic lipid abnormalities, in addition to insulin resistance, ligands are required that can activate both PPARa and PPARg. In this study, we used chimeric PPARa/g reporter...

We have investigated the effect of the soybean isoflavone genistein on 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in calf thymus DNA exposed to either UV irradiation or the Fenton reaction system. Under the conditions used we observed that UV light and the Fenton reaction significantly increase 8-OHdG formation in DNA. Co-incubation with genistein inhibits the formation of 8-OHdG induced by...

introduction: non-enzymatic glycosylation of proteins which is the major cause of diabetic complications, cardiovascular diseases retinopathy, nephropathy and neuropathy can effectively be inhibted by antioxidants. materials and method: the antioxidant effects of several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin a were studied  on glycosylation of...

most common cause of age-related bone loss. Hormone replacement therapy (HRT) can resolve most postmenopausal problems. However, compliance with HRT is poor because of risks of breast and endometrial cancers associated with longterm use of HRT. In an effort to search for an alternative treatment, potential health benefits of phytoestrogens have been suggested. Phytoestrogens are naturally occur...

Confocal fluorescence microscopy have been employed to investigate phase separation in giant unilamellar vesicles prepared from binary mixtures of unsaturated dioleoylphosphocholine with saturated phosphocholines or brain sphingomyelin in the absence and presence of the flavonoids, biochanin A, phloretin, and myricetin. It has been demonstrated that biochanin A and phloretin make uncolored doma...

1,1mg I plus 0,42 mg DL-Phenylalanin-(l-14C) wur­ den in 15 cm3 Wasser gelöst. 1.22 mg II wurden in 15 cm3 Wasser mit 0,5 cm3 7i/lOO-NaOH in das wasser­ lösliche Mono-Natriumsalz überführt und 0,42 mg d l Phenylalanin-(l-14C) zugefügt. Versuchsdauer 48 Stun­ den. Die Isolierung und Reinigung der Isoflavone er­ folgte wie im vorigen Versuch. Ausbeuten: Versuch 1. 4,8 mg Biochanin-A und 1,88 mg F...

Flavonoids are constituents of fruits, vegetables, and plant-derived beverages, as well as components in herbal-containing dietary supplements. The objective of this investigation was to characterize the effect of flavonoids on P-glycoprotein (P-gp)-mediated cellular efflux and to determine the molecular mechanism(s) of the flavonoid-drug interaction. Studies were conducted in the sensitive and...

Increasing evidences show that the etiology of Parkinson's disease (PD) is multifactorial. Studying the combined effect of several factors is becoming a hot topic in PD research. On one hand, iron is one of the essential trace metals for human body; on the other hand, iron may be involved in the etiopathogenesis of PD. In our present study, the rats with increased neonatal iron (120 μg/g bodywe...