Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are released by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohne...

The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commerciall...

An optimization and comparison of trypsin digestion strategies for peptide/protein identifications by microLC-MS/MS with or without MS compatible detergents in mixed organic-aqueous and aqueous systems was carried out in this study. We determine that adding MS-compatible detergents to proteolytic digestion protocols dramatically increases peptide and protein identifications in complex protein m...

As anyone who has ever blown bubbles as or with a child knows, if you stop blowing before the sphere is complete, it collapses and disappears. The gas exchange surfaces of our lungs, the alveoli, are also highly curved, thin-walled, moist membranes that are open to the atmosphere. They too, would tend to go poof! and disappear, save for a mixture of phospholipids and proteins that lines the epi...

Aims: T1 /(podoplanin) is abundantly expressed in the alveolar epithelial type I cells (ATI) of rodent and human lungs. Caveolin-1 is a classical primary structural protein of plasmalemal invaginations, so-called caveolae, which represent specialized lipid rafts, and which are particularly abundant in ATI cells. The biological functions of T1 in the alveolar epithelium are unknown. Here we repo...

1. The bactericidal action of a number of anionic and cationic synthetic detergents on four Gram-positive and three Gram-negative bacteria has been investigated. 2. Cationic detergents, as a group, were found to exhibit marked bactericidal effects on Gram-positive microorganisms and somewhat less pronounced action on Gram-negative organisms. 3. The anionic detergents were germicidal only agains...

the effect of triton x-100, na cholate and tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of salmonella typhimurium was studied. the detergents were used in various concentrations (1.6 to 64 mm) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. results showed that no inne...