Article Type: Full Length Research Article Pseudomonas putida HK-44, a bioluminescent reporter strain with a nah-lux fusion, can be used to assess the presence of polynuclear aromatic hydrocarbons by stimulating the light production upon inducing the naphthalene dioxygenase system. However, this assay can be affected by the presence of oxygen. The aim of this work was to evaluate the effect of ...

Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number of drugs for treatment are available. A screening method for new antifungal compounds is urgently required, preferably an approach suitable for in vitro and in vivo studies. Bioluminescence imaging is a powerful tool to study the temporal and spatial resolutions of the infe...

Under physiological conditions firefly luciferase catalyzes the highly efficient emission of yellow-green light from the substrates luciferin, Mg-ATP, and oxygen. In nature, bioluminescence emission by beetle luciferases is observed in colors ranging from green (approximately 530 nm) to red (approximately 635 nm), yet all known luciferases use the same luciferin substrate. In an earlier report ...

In this study, we attempted to control the timing of light-emission from bioluminescent bacteria, by changed cell numbers inoculated into medium. Luminous bacteria express bioluminescence when the number of cells reached a threshold. Inoculated cell density had an effect on the time of bioluminescence starting. Samples were prepared by varying cell density of inoculation. In the results, all th...

BACKGROUND The porcine oocyte maturation in vivo occurs within the ovarian follicle and is regulated by the interactions between oocytes and surrounding follicular components, including theca, granulosa, and cumulus cells, and follicular fluid. Therefore, the antral follicle is an essential microenvironment for efficient oocyte maturation and its developmental competence. Quantitative biolumine...

Spectrally-resolved BioLuminescence optical Tomography (sBLT) is used to recover images of Luciferase activity within a 3D model using multi-wavelength emission data from internal bioluminescence sources. The Adjoint theorem for 3D image reconstruction is presented and validated using experimental data with a complete computation time of 5 minutes. ©2006 Optical Society of America OCIS codes: (...

Spectrally-resolved BioLuminescence optical Tomography (sBLT) is used to recover images of Luciferase activity within a 3D mouse model using multi-wavelength emission data from multiple internal bioluminescence sources. The images show that the location of sources can be reconstructed accurately using a fast, direct and linear reconstruction algorithm. ©2006 Optical Society of America OCIS code...

In diabetes research, bioluminescence imaging (BLI) has been applied in studies of β-cell impairment, development, and islet transplantation. To develop a mouse model that enables noninvasive imaging of β cells, we generated a bacterial artificial chromosome (BAC) transgenic mouse in which a mouse 200-kbp genomic fragment comprising the insulin I gene drives luciferase expression (Ins1-luc BAC ...

We present an optimized triple modality reporter construct combining a far-red fluorescent protein (E2-Crimson), enhanced firefly luciferase enzyme (Luc2), and truncated wild type herpes simplex virus I thymidine kinase (wttk) that allows for sensitive, long-term tracking of tumor growth in vivo by fluorescence, bioluminescence, and positron emission tomography. Two human cancer cell lines (MDA...

Bioluminescence is a natural light emitting phenomenon that is emitted by the several terrestrial, marine and some fresh water organisms. This phenomenon could be found in all the major taxonomic groups that are known to comprise around 700 genera of bioluminescent organisms. Though their luminescence emissions are due to two principal components i.e. luciferin and luciferase, yet for many biol...