Salvia miltiorrhiza Bunge is an important traditional Chinese medicine (TCM). In this study, two S. miltiorrhiza genotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. There were 670 differentially expressed genes (DEGs) identified between BH18 and ZH23, 250 of which ...

The tetrapyrrole biosynthetic pathway provides the vital cofactors and pigments for photoautotrophic growth (chlorophyll), several essential redox reactions in electron transport chains (haem), N- and S-assimilation (sirohaem), and photomorphogenic processes (phytochromobilin). While the biochemistry of the pathway is well understood and almost all genes encoding enzymes of tetrapyrrole biosynt...

Expression of foreign proteins in E. coli is normally inhibited by exogenous production of acetate. To overcomethis problem, various strategies have been proposed and tested to reduce the extent of acetate accumulation.Although these strategies can improve the outcome, the implementation of their proposed techniquesis not practical. Because to achieve optimal results, it requi...

under osmotic stresses, proline accumulation is an important response of plants to these conditions. proline is a compatible osmolyte which affects many cellular and molecular aspects of plant in both normal and stressful situations. proline is shown to be involved in plant development in normal condition and in conferring resistance to plant under biotic and abiotic stresses. therefore, many s...

Ginkgo biloba leaves contain abundant flavonoids, and flavonoid accumulation is affected by age. MicroRNAs (miRNAs) play an important role in the plant aging pathway. However, miRNAs involved biosynthesis related to age G. have rarely been studied. In this study, we compared 1-, 4-, 7-year-old ginkgo seedings found a significant decrease content of quercetin, kaempferol, total flavonol aglycone...

The fungus Cercospora rosicola produces abscisic acid (ABA) as a secondary metabolite. We developed a convenient system using this fungus to determine the effects of compounds on the biosynthesis of ABA. Inasmuch as ABA and the gibberellins (GAs) both arise via the isoprenoid pathway, it was of interest to determine if inhibitors of GA biosynthesis affect ABA biosynthesis. All five putative inh...