The LmR1 locus, which controls seedling resistance to the blackleg fungus Leptosphaeria maculans in the Brassica napus cultivar Shiralee, was positioned on linkage group N7. Fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with LmR1. Additional linkage mapping in a second population colocalized a seedling resistance gene, ClmR1, f...

Pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. Here, we report the draft genome sequences of three weakly virulent P. carotovurum subsp. brasiliense strains isolated in Canada. Analysis of these genome sequences will help to pinpoint differences in virulence among P. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate re...

Blackleg, caused by Leptosphaeria maculans, is a major threat to canola production in Canada. With the exception of China, L. maculans is present in areas around the world where cruciferous crops are grown. The pathogen can cause trade barriers in international canola seed export due to its potential risk as a seed contaminant. The most recent example is China restricting canola seeds imported ...

The fungus Leptosphaeria maculans (L. maculans) is the causal agent of blackleg disease of canola/oilseed rape (Brassica napus) worldwide. We previously reported cloning of the B. napus blackleg resistance gene, LepR3, which encodes a receptor-like protein. LepR3 triggers localized cell death upon recognition of its cognate Avr protein, AvrLm1. Here, we exploited the Nicotiana benthamiana model...

The term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. In this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16S ribosomal DNA directly amplified from plant tissue DNA was used in combination ...

Canola (Brassica napus L.) is the second largest cash crop in Canada (4). Blackleg disease, caused by Leptosphaeria maculans (Desmaz.) Ces. & De Not. (anamorph = Phoma lingam (Tode:Fr.) Desmaz.), is one of most economically important diseases of canola (21). In the past decade, canola production has expanded significantly worldwide and interest in the blackleg fungus, particularly its biology, ...

The effects of a 4-year crop rotation and two tillage systems on blackleg (Leptosphaeria maculans) disease of canola were determined in a field experiment carried out from 1999 through 2002 at Carman, Manitoba. Crops in rotation were canola (Brassica napus) (C), wheat (Triticum aestivum) (W), and flax (Linum usitatissimum) (F). Rotations were done under conventional tillage (T) or zero-till (Z)...