Western blotting is a commonly used technique in biological research. A major problem with Western blotting is not the method itself, but the use of poor quality antibodies as well as the use of different experimental conditions that affect the linearity and sensitivity of the Western blot. Investigation of some conditions that are commonly used and often modified in Western blotting, as well a...

This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...

ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. enormous economic losses annually occur in livestock production around the world as a result oftheir existance. one of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. for identification of the putative protec...