Bombyx mori nucleopolyhedrovirus (BmNPV) that infects the silkworm, B. mori, accounts for >50% of silk cocoon crop losses globally. We speculated that simultaneous targeting of several BmNPV essential genes in transgenic silkworm would elicit a stable defense against the virus. We introduced into the silkworm germline the vectors carrying short sequences of four essential BmNPV genes in tandem,...

in mulberry orchards in northern part of iran, thrips is a dominant pest and causing a lot of damage to the mulberry. the effect of pest on bio-chemical parameters of mulberry leaf of two varieties viz. kenmochi and ichenoise and also the effect of feeding of infested leaves on rearing performances have been studied. for the study, six plots of 200 m2 area of both the varieties were selected an...

A bivoltine silkworm hybrid, KA x NB4D2 was treated with the juvenoid R394 (Ethyl-9 cyclohexyl-3,7-dimethyl-2,4-nonadienoate) at a dose of 0.039 nl/larva at 24, 48, 72 and 96 h of 5th instar for silk yield improvement. Treatment specific significant increase was observed in the cocoon and shell weight with no such marked variation in the shell percentage. Notable changes were also seen in tr...

Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence contains an open reading frame of 783 bp encoding a protein of 260 amino acids with a predicted mo...

oral supplementation with 50, 100 and 150 ?g/ml calcium chloride to the fifth stadium larvae of csr2xcsr4 biovoltine hybrid silkworm, b. mori on fat body glycogen, protein, and total lipids and haemolymph trehalose and protein have been studied. one of the four normal feeds per day was substituted with treated leaves fed to silkworms at fifth instar larvae. the controls were fed with the leaves...

the domesticated silkworm, bombyx mori, is of high commercial importance as a silk producer and is also widely used for implementation of basic and applied research. it is important to understand its genome organization using molecular markers for genetic studies and for breeding purposes. in this study, a genetic linkage map using 204 amplified fragment length polymorphism (aflp) markers was d...

ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performe...

Bombyx mori nucleopolyhedrovirus (BmNPV) is a typical species of Baculoviridae. The complete genome sequence of a BmNPV strain with cubic occlusion bodies is reported here. The genome of this strain consists of 127,465 nucleotides with a G+C content of 40.36% and is 97.3% and 97.5% identical to those of BmNPV strain T3 and Bombyx mandarina NPV S1, respectively. Despite the abnormal polyhedra it...

Abstract The structural and thermal properties of chitin chitosan isolated from different sources ( Artemia sp.cysts (from the Aral Sea) Silkworm pupae (Bombyx mori) ) were studied by using FTIR, XRD, DSC. It is shown, that samples have a similar structure, however, depending on differ in ratio phase composition. Two types crystalline modification structure α -and β-modifications are observed f...