The rapid progression of multiple myeloma is dependent upon cellular interactions within the bone marrow microenvironment. In vitro studies suggest that bone marrow stromal cells (BMSC) can promote myeloma growth and survival and osteolytic bone disease. However, it is not possible to recreate all cellular aspects of the bone marrow microenvironment in an in vitro system, and the contributions ...

BACKGROUND AIMS Neural stem cells (NSC) derived from bone marrow stromal cells (BMSC) (BMSC-D-NSC) are remarkably versatile in response to environmental signals, which render them useful in the search for neurodegenerative disease treatments. METHODS We isolated NSC from rhesus monkey bone marrow (BM), transfected them with the human tyrosine hydroxylase (hTH) gene, and transplanted them into...

The possibility of using adipose tissue-derived stromal cells (ATSC) as alternatives to bone marrow-derived stromal cells (BMSC) for bone repair has garnered interest due to the accessibility, high cell yield, and rapid in vitro expansion of ATSC. For clinical relevance, their bone forming potential in comparison to BMSC must be proven. Distinct differences between ATSC and BMSC have been obser...

(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method the assembly of arrays microtissues from stromal cells (BMSC) in vitro and their maturation into micro-ossicles vivo. (2) Methods: Discs with 50 microwells were used to assemble 3 × 105 BMSCs each on nylon mesh carrier. Microtissues cultured chondrogenic ind...

Background: The present study investigated the functional maturity of oligodendrocyte derived from rat bone marrow stromal cells (BMSC). Methods: The BMSC were isolated from female Sprague-Dawley rats and evaluated for different markers, such as fibronectin, CD106, CD90, Oct-4 and CD45. Transdifferentiation of OLC from BMSC was obtained by exposing the BMSC to DMSO and 1 µM all-trans-retinoic a...

Objective- Adipose tissue is an appropriate source for isolation of cells with stem-cell–like properties.  In the present long-term study, the effects of the omental adipose-derived nucleated cells (OADNCs) fraction were compared to those of the undifferentiated cultured bone marrow stromal cells (BMSCs) on sciatic nerve regeneration. Design- Experimental in vivo study. Animals- Fift...

We characterized bone marrow stromal cells (BMSC) from patients with pre-fibrotic myeloproliferative neoplasms (MPN). MPN-BMSC showed decreased capacity to stimulate the proliferation of colony-forming units of normal hematopoietic stem and progenitor cells and displayed increased matrix remodelling (in particular fibronectin deposition) compared to control BMSC. This finding was confirmed in p...

Secretomes of mesenchymal stromal cells (MSCs) are emerging as a novel growth factor (GF)-based strategy for periodontal and bone regeneration. The objective this study was to compare the secretome human marrow MSC (BMSC) that leukocyte- platelet-rich fibrin (L-PRF), an established GF-based therapy, in context wound healing Conditioned media from BMSCs (BMSC-CM) L-PRF (LPRF-CM) were subjected q...

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...