Coupling capillary columns in gas chromatography (GC) is not an operation that is only relevant for the users of the most advanced capillary GC methods. Simple single-column separations can also benefit from a properly selected and installed pre-column. Pre-columns connected upstream of the main column can increase the life-time of the column, efficiently refocus the analyte bands to result in ...

Ionic analytes, such as peptides, can be challenging to separate by reverse-phase chromatography with optimal efficiency. They tend, for instance, to exhibit poor peak shapes, particularly when eluted with mobile phases preferred for electrospray ionization mass spectrometry. We demonstrate that a novel charged-surface C18 stationary phase alleviates some of the challenges associated with rever...

Amrinone (AMR), a bipyridine derivative, is receiving increasing use in postoperative cardiac patients as an inotrope and vasodilator. The hemodynamic response to amrinone in adults is linearly related to AMR concentrations, warranting therapeutic drug monitoring. We report a rapid microsample HPLC method for monitoring AMR and its principal metabolites, N-acetyl (N-ac) and N-glycolyl (N-gly) A...

Proteomic samples such as human serum are an extreme challenge to study due to the large dynamic range of the proteins of interest. The proteins in human serum can range from those considered “high-abundance” proteins, present at 2%–50% of the total protein mass (typically the μg/mL–mg/mL range) to those considered tissue biomarkers present in the pg/mL–ng/mL range. Multidimensional separation ...

A tidal bore is a surge of water propagating upstream as the tidal flow turns to rising into an estuary with a tidal range larger than 5 to 6 m and a bathymetry that amplifies the tidal wave. The bore front is a shock characterised by a singularity of the free-surface and pressure and velocity fields. This study aims to characterise the tidal bore propagation and the induced turbulent mixing un...

A simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The chromatographic ...

This paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products (special food intended for specific population groups). All four compounds are soluble in aqueous solutions and can easily be separated and ...