The main bacterium associated with skin infection is Staphylococcus aureus, occurring especially in infections acquired via surgical wounds, commonly leading to lethal hospital-acquired infections, emphasizing the importance of identifying new antimicrobial compounds. Among them, cyclotides have gained interest due to their high stability and multifunctional properties. Here, cycloviolacin 2 (C...

Objective To conduct a trial of the effectiveness of magnetic bracelets as used in the consumer market for pain control in osteoarthritis of hip and knee. Design Randomised, placebo-controlled trial with three parallel groups. Setting Primary care study set in five rural general practices in Mid Devon, UK. Subjects 194 men and women aged 45 to 80 years with osteoarthritis of the hip or knee. In...

Much interest has been generated by recent reports on the discovery of circular (i.e. head-to-tail cyclized) proteins in plants. Here we report the three-dimensional structure of one of the newest such circular proteins, MCoTI-II, a novel trypsin inhibitor from Momordica cochinchinensis, a member of the Cucurbitaceae plant family. The structure consists of a small beta-sheet, several turns, and...

BACKGROUND Cyclotides are useful scaffolds to stabilize bioactive peptides. RESULTS Four melanocortin analogues of kalata B1 were synthesized. One is a selective MC4R agonist. CONCLUSION The analogues retain the native kalata B1 scaffold and introduce a designed pharmacological activity, validating cyclotides as protein engineering scaffolds. SIGNIFICANCE A novel type of melanocortin agon...

Cyclotides as a cyclic peptide produced by different groups of plants have been very attractive field research due to their exceptional properties in biological activities and drug design applications. The importance cyclotides new from nature caused attract researchers develop separation systems. Recent growth development on chip-based technology for bioassay especially anticancer having spark...

Cyclotides are plant-derived proteins that have a unique cyclic cystine knot topology and are remarkably stable. Their natural function is host defense, but they have a diverse range of pharmaceutically important activities, including uterotonic activity and anti-HIV activity, and have also attracted recent interest as templates in drug design. Here we report an unusual biosynthetic origin of a...

Cyclotides are head-to-tail cyclized peptides comprising a stabilizing cystine-knot motif. To date, they are well known for their diverse bioactivities such as anti-HIV and immunosuppressive properties. Yet little is known about specific molecular mechanisms, in particular the interaction of cyclotides with cellular protein targets. Native and synthetic cyclotide-like peptides from Momordica pl...

Cyclotides are a family of macrocyclic peptides that combine the unique features of a head-to-tail cyclic backbone and a cystine knot motif, the combination of which imparts them with extraordinary stability. The prototypic cyclotide kalata B1 is toxic against two economically important gastrointestinal nematode parasites of sheep, Haemonchus contortus and Trichostrongylus colubriformis. A lysi...